Thyrocyte-derived exosome-targeted dendritic cells stimulate strong CD4+ T lymphocyte responses. (15th April 2020)

• Record Type: Journal Article
• Authors: Cui, Xuejiao; Wang, Shuo; Zhao, Na; Wang, Shiwei; Wang, Zhenzhen; Huang, Mingshi; Liu, Yongping; Qin, Jing; Shan, Zhongyan; Teng, Weiping; Li, Yushu
• Is Part Of: Molecular and cellular endocrinology. Volume 506(2020)
• Publication Date: 2020-04-15
• Subjects: Thyroid cell -- Exosomes -- Dendritic cell -- T lymphocyte -- Hashimoto thyroiditis; Endocrinology -- Periodicals; Molecular biology -- Periodicals; Cytology -- Periodicals; Endocrinology -- Periodicals; Hormones -- Periodicals; Endocrinologie -- Périodiques; Cytology; Endocrinology; Molecular biology; Periodicals; 573.4
• DOI: 10.1016/j.mce.2020.110756
• Languages: English
• ISSNs: 0303-7207

Abstract

Abstract: Exosomes have been intensively studied in autoimmune diseases, and circulating exosomes and microvesicles have also been explored in autoimmune thyroiditis (AITD). However, the role of thyroid cell-derived exosomes in immune responses is unclear. We showed that IFN-γ-treated Nthy-ori 3-1 cell-derived exosomes (IFN-γ-Exo) harbored TPO, HSP60 and MHC-II and activated dendritic cells (DCs) in vitro. Compared with Exo-targeted DCs (DCExo ), IFN-γ-Exo-targeted DCs (DCIFN-γ-Exo ) promoted the expression and release of proinflammatory cytokines, such as IFN-γ, IL-17A and IL-22, from CD4 + T lymphocytes and inhibited the expression and release of anti-inflammatory cytokines, such as IL-4, IL-10 and TGF-β1; however, IFN-γ-Exo did not have this effect compared with Nthy-ori 3-1 cell-derived exosomes (Exo). DCIFN-γ-Exo stimulates the expression and release of cytokines from CD4 + T lymphocytes more efficiently than IFN-γ-Exo. Thus, DCIFN-γ-Exo may effectively induce CD4 + T lymphocyte-mediated immune responses and play a role in the occurrence and development of AITD. Highlights: IFN-γ-treated thyroid cell-derived exosomes (IFN-γ-Exo) express TPO, HSP60, MHC-II. IFN-γ-Exo activate dendritic cells (DCs). IFN-γ-Exo need to activate DCs before triggering CD4 + T lymphocyte differentiation.