Immunohistochemical Detection of γ/δ T Lymphocytes in Formalin-fixed Paraffin-embedded Tissues. Issue 8 (September 2019)

• Record Type: Journal Article
• Authors: Jungbluth, Achim A.; Frosina, Denise; Fayad, Miriam; Pulitzer, Melissa P.; Dogan, Ahmet; Busam, Klaus J.; Imai, Naoko; Gnjatic, Sacha
• Is Part Of: Applied immunohistochemistry & molecular morphology. Volume 27:Issue 8(2019)
• Publication Date: 2019-09
• Subjects: TCRγ/δ -- TCRα/β -- T lymphocytes -- immunohistochemistry -- paraffin-embedded tissue; Diagnostic immunohistochemistry -- Periodicals; Immunohistochemistry -- Periodicals; Cells -- Morphology -- Periodicals; Molecular diagnosis -- Periodicals; 616.079
• DOI: 10.1097/PAI.0000000000000650
• Languages: English
• ISSNs: 1541-2016

Abstract

Abstract : T lymphocytes can be distinguished based on the composition of the T-cell receptor (TCR) chain in α/β T cells and γ/δ T cells. Correspondingly, α/β lymphomas can be distinguished from γ/δ lymphomas. The latter are rare neoplasms, which are usually confined to particular organs and tissues and carry a dismal prognosis. Until recently, monoclonal antibody (mAb) clone g3.20 to the TCR γ-chain was the reagent of choice for the immunohistochemical detection of γ/δ T cells and lymphomas in standard formalin-fixed paraffin-embedded tissues. However, due to technical problems, mAb g3.20 became recently unavailable. Our attempts to identify another commercially available clone to the TCR γ-chain were unsuccessful. However, we were able to identify a mAb (clone H-41, SC-100289; Santa Cruz, Dallas, TX) to the TCR δ-chain. H-41 works well in immunohistochemistry on paraffin-embedded tissue and comparison with previously stained cases, shows superior immunolabeling to mAb g3.20. H-41 to the TCR δ-chain appears to be a suitable reagent for the replacement of mAb g3.20.