Human tuberculosis brain promotes neuronal apoptosis but not in astrocytes with high expression of vascular endothelial growth factor. (September 2018)

• Record Type: Journal Article
• Authors: Othman, Fatihah N.; Muthuraju, Sangu; Noor, Siti Suraiya M.; Abdullah, Sarimah; Mohd Yusoff, Abdul Aziz; Tharakan, John; Bhaskar, Shalini; Mahmood, Mohd Shah; Kassim, Fauziah; Rafia, Hanip; Mohd Haspani, Mohd Saffari; Alias, Azmi; Pando, Rogelio H.; Abdullah, Jafri M.; Jaafar, Hasnan
• Is Part Of: Tuberculosis. Volume 112(2018)
• Page Start: 45
• Page End: 51
• Publication Date: 2018-09
• Subjects: Human brain tuberculosis -- Astrocyte -- Neurons -- Apoptosis -- VEGF -- Bcl2 and Bax; 616.995
• DOI: 10.1016/j.tube.2018.07.007
• Languages: English
• ISSNs: 1472-9792

Abstract

Abstract: The present study aimed to investigate the involvement of the angiogenic marker vascular endothelia growth factor (VEGF) and apoptotic markers of Bcl-2 and Bax in the neurons and astrocytes in the brain infected by Mycobacterium tuberculosis . The immunohistochemistry staining was performed to analyze the expression of the VEGF, Bcl-2 and Bax in the astrocytes and neurons. The expression of VEGF was high in neurons and astrocytes in both the infected brain and control tissues with no difference of angiogenic activity (p = 0.40). Higher Bcl-2 expression was seen in astrocytes of infected brain tissues compared to the control tissues (p = 0.004) promoted a higher anti-apoptotic activity in astrocytes. The neurons expressed strong Bax expression in the infected brain tissues compared to the control tissues (p < 0.001), which indicated more apoptosis in neurons. Thus, neuronal death and survival of infected astrocytes together with high expression of VEGF might be associated with formation of brain tuberculosis. In conclusion, neurons could be more vulnerable than astrocytes in human tuberculosis brain with high expression of VEGF.