Litopenaeus vannamei fed diets with different replacement levels of fish meal by fish silage: A molecular approach on intestinal microbiota. (29th March 2019)

• Record Type: Journal Article
• Authors: Shao, Jianchun; Jiang, Keyong; Wang, Lei
• Is Part Of: Aquaculture nutrition. Volume 25:Number 3(2019)
• Page Start: 721
• Page End: 728
• Publication Date: 2019-03-29
• Subjects: composition -- diversity -- growth -- high‐throughput sequencing -- metabolism function -- shrimp; Aquaculture -- Periodicals; Aquatic animals -- Feeding and feeds -- Periodicals; Fishes -- Feeding and feeds -- Periodicals; 639.3
• DOI: 10.1111/anu.12900
• Languages: English
• ISSNs: 1353-5773

Abstract

Abstract: We investigated the intestinal microbiota in shrimp fed diets with five replacement levels (0, 25%, 50%, 75% and 100%) of fish meal by fish silage. An 8‐week feeding trail was conducted in 15 fibreglass tanks (400 L volume, three replications for each diet) with 50 shrimps (initial weight 0.26 ± 0.03 g) per tank. After 8‐week trial, shrimp fed diets with low replacement levels (0% and 25%) of fish meal by fish silage had significantly enhanced growth performance ( p < 0.05). Based on the Illumina sequencing of 15 samples, a total of 5, 778 OTUs were obtained and the number of OTUs in each sample ranged from 2, 346 to 3, 491. OTUs were identified into 23 phyla, and Proteobacteria was the most abundant. At family and genus levels, family Vibrionaceae and genus Photobacterium were more abundant in high replacement level groups (50%, 75% and 100%) than those in low replacement level groups (0% and 25%), which may be related to the worse growth performance of high replacement level groups. However, dietary different levels of fish silage did not make significant difference on intestinal bacterial richness, diversity and metabolism functions ( p > 0.05), which indicated the limited impact of dietary fish silage on intestinal microbiota.