Characterization of a long noncoding RNA Pcdh17it as a novel marker for immature premyelinating oligodendrocytes. Issue 11 (22nd July 2019)

• Record Type: Journal Article
• Authors: Kasuga, Yusuke; Fudge, Alexander D.; Zhang, Yumeng; Li, Huiliang
• Other Names: Fields R. Douglas guestEditor.; Richardson William D. guestEditor.
• Is Part Of: Glia. Volume 67:Issue 11(2019)
• Page Start: 2166
• Page End: 2177
• Publication Date: 2019-07-22
• Subjects: immature oligodendrocyte -- long noncoding RNA -- oligodendrocyte -- oligodendrocyte dynamics -- Pcdh17it -- premyelinating oligodendrocyte; Neuroglia -- Periodicals; Neurology -- Periodicals; 611.0188
• DOI: 10.1002/glia.23684
• Languages: English
• ISSNs: 0894-1491

Abstract

Abstract: Oligodendrocyte precursors (OPs) proliferate and differentiate into oligodendrocytes (OLs) during postnatal development and into adulthood in the central nervous system (CNS). Following the initiation of differentiation, OPs give rise to immature, premyelinating OLs, which undergo further differentiation and mature into myelin‐forming OLs. We identified an immature OL‐specific long noncoding RNA, named Pcdh17it . Through co‐localization analysis and morphological characterization of OLs, we found that Pcdh17it is a specific marker for newly born immature OLs in the developing and adult forebrain of mice, and we used this new marker to analyze OL generation over the lifespan of mice. Pcdh17it is an effective tool for monitoring newly born OLs in adult brain, allowing detailed study of the dynamics of OP differentiation into OLs in the normal and pathological CNS. Main points: Pcdh17it specifically marks newly born immature oligodendrocytes (OLs). Pcdh17it is an effective tool for studying OL dynamics in situ. Pcdh17it helps subdivide immature OLs into two distinct developmental stages, "small spidery" and "large spidery".