Self‐Immobilizing Oxidoreductases for Flow Biocatalysis in Miniaturized Packed‐Bed Reactors. Issue 10 (21st June 2019)

• Record Type: Journal Article
• Authors: Peschke, Theo; Bitterwolf, Patrick; Rabe, Kersten S.; Niemeyer, Christof M.
• Other Names: Dittmeyer Roland guestEditor.
• Is Part Of: Chemical engineering & technology. Volume 42:Issue 10(2019)
• Page Start: 2009
• Page End: 2017
• Publication Date: 2019-06-21
• Subjects: Biocatalysis -- Enzymes -- Immobilization techniques -- Microreactors -- Stereoselective reactions; Chemical engineering -- Periodicals; Chemical processes -- Periodicals; 660.05
• DOI: 10.1002/ceat.201900073
• Languages: English
• ISSNs: 0930-7516

Abstract

Abstract: The industrial implementation of enzymes in flow biocatalysis microreactors is expected to be essential for the emergence of a bio‐based circular economy. Major challenges concern the efficient immobilization of delicate enzymes inside miniaturized reactors without compromising their catalytic activity. We describe the exploitation of the widely used His‐tag system in a microfluidic packed‐bed reactor that contains ketoreductase‐functionalized magnetic beads. In a continuous process, these reactors produced highly stereoselective ( R )‐configured alcohols (d.r. 99:1) with an average conversion of > 90 % for more than 4 days. We believe that such miniaturized flow reactors can be of great utility for future sustainable production processes. Abstract : Flow biocatalysis requires the retention of enzymes inside the flow reactors. To meet this demand, two self‐immobilizing oxidoreductases (red and blue) were immobilized via a genetically encoded His‐tag on Co 2+ ‐functionalized magnetic microbeads (brown) that can be loaded into miniaturized packed‐bed reactors to facilitate continuous production of chiral alcohols.