Long‐term expanding human airway organoids for disease modeling. (14th January 2019)

• Record Type: Journal Article
• Authors: Sachs, Norman; Papaspyropoulos, Angelos; Zomer‐van Ommen, Domenique D; Heo, Inha; Böttinger, Lena; Klay, Dymph; Weeber, Fleur; Huelsz‐Prince, Guizela; Iakobachvili, Nino; Amatngalim, Gimano D; de Ligt, Joep; van Hoeck, Arne; Proost, Natalie; Viveen, Marco C; Lyubimova, Anna; Teeven, Luc; Derakhshan, Sepideh; Korving, Jeroen; Begthel, Harry; Dekkers, Johanna F; Kumawat, Kuldeep; Ramos, Emilio; van Oosterhout, Matthijs FM; Offerhaus, G Johan; Wiener, Dominique J; Olimpio, Eduardo P; Dijkstra, Krijn K; Smit, Egbert F; van der Linden, Maarten; Jaksani, Sridevi; van de Ven, Marieke; Jonkers, Jos; Rios, Anne C; Voest, Emile E; van Moorsel, Coline HM; van der Ent, Cornelis K; Cuppen, Edwin; van Oudenaarden, Alexander; Coenjaerts, Frank E; Meyaard, Linde; Bont, Louis J; Peters, Peter J; Tans, Sander J; van Zon, Jeroen S; Boj, Sylvia F; Vries, Robert G; Beekman, Jeffrey M; Clevers, Hans;
• Is Part Of: EMBO journal. Volume 38:Number 4(2019)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2019-01-14
• Subjects: 3D culture -- airway organoids -- cystic fibrosis -- lung cancer -- respiratory syncytial virus; Molecular biology -- Periodicals; 572.805
• DOI: 10.15252/embj.2018100300
• Languages: English
• ISSNs: 0261-4189

Abstract

Abstract: Organoids are self‐organizing 3D structures grown from stem cells that recapitulate essential aspects of organ structure and function. Here, we describe a method to establish long‐term‐expanding human airway organoids from broncho‐alveolar resections or lavage material. The pseudostratified airway organoids consist of basal cells, functional multi‐ciliated cells, mucus‐producing secretory cells, and CC10‐secreting club cells. Airway organoids derived from cystic fibrosis (CF) patients allow assessment of CFTR function in an organoid swelling assay. Organoids established from lung cancer resections and metastasis biopsies retain tumor histopathology as well as cancer gene mutations and are amenable to drug screening. Respiratory syncytial virus (RSV) infection recapitulates central disease features, dramatically increases organoid cell motility via the non‐structural viral NS2 protein, and preferentially recruits neutrophils upon co‐culturing. We conclude that human airway organoids represent versatile models for the in vitro study of hereditary, malignant, and infectious pulmonary disease. Synopsis: To date, persistent in vitro culture of adult human lung epithelium remains elusive. In this methods resource article, culture conditions to maintain three‐dimensional pulmonary tissue long‐term are reported and applied to recapitulate related diseases. Culture conditions for long‐term expansion of healthy, hereditary disease and malignant human airway epithelial organoids. Airway organoids are amenable for medium‐throughput drug screening. Airway organoids readily allow modeling of viral infection. Abstract : Three‐dimensional human pulmonary tissue culture allows for investigation of hereditary diseases.