When will my mouse die? Life span prediction based on immune function, redox and behavioural parameters in female mice at the adult age. (September 2019)

• Record Type: Journal Article
• Authors: Martínez de Toda, Irene; Vida, Carmen; Sanz San Miguel, Luis; De la Fuente, Mónica
• Is Part Of: Mechanisms of ageing and development. Volume 182(2019)
• Publication Date: 2019-09
• Subjects: AGEs Advanced Glycated End-products -- BCA Bicinchoninic Acid -- Con A Concanavalin A -- EPM Elevated Plus Maze -- GPx Glutathione Peroxidase -- GR Glutathione Reductase -- GSH Reduced Glutathione -- GSSG Glutathione disulfide -- HB Hole board -- IL Interleukin -- MDA Malondialdehyde -- MLR Multiple Linear Regression -- NADPH Reduced Nicotinamide Adenine Dinucleotide Phosphate -- NBT Nitro Blue Tetrazolium -- NF-kB Nuclear Factor Kappa B -- OPT o-phthalaldehyde -- ROS Reactive Oxygen Species -- TBA Thiobarbituric Acid -- TNF-α Tumour necrosis factor-α; Life span prediction -- Multiple linear regression -- Immune function -- Oxidative stress -- Anxiety-like behaviour; Aging -- Periodicals; Developmental biology -- Periodicals; Aging -- Periodicals; Developmental Biology -- Periodicals; Vieillissement -- Périodiques; Biologie du développement -- Périodiques; Aging; Developmental biology; Periodicals; 612.67
• DOI: 10.1016/j.mad.2019.111125
• Languages: English
• ISSNs: 0047-6374

Abstract

Highlights: Macrophage functionality and lymphoproliferation are predictive of life span. Glutathione, malondialdehyde and glutathione peroxidase are predictive of life span. Internal locomotion and time in open arms indices are predictive of life span. Immunity, Redox, Behavioural and Immunity-Redox models have been cross-validated. Those with best reproducibility were the Immunity and the Immunity-Redox models. Abstract: The identification of predictive markers of life span would help to unravel the underlying mechanisms influencing ageing and longevity. For this aim, 30 variables including immune functions, inflammatory-oxidative stress state and behavioural characteristics were investigated in ICR-CD1 female mice at the adult age (N = 38). Mice were monitored individually until they died and individual life spans were registered. Multiple linear regression was carried out to construct an Immunity model (adjusted R 2 = 75.8%) comprising Macrophage chemotaxis and phagocytosis and Lymphoproliferation capacity, a Redox model (adjusted R 2 = 84.4%) involving Reduced Glutathione and Malondialdehyde concentrations and Glutathione Peroxidase activity and a Behavioural model (adjusted R 2 = 79.8%) comprising Internal Locomotion and Time spent in open arms indices. In addition, a Combined model (adjusted R 2 = 92.4%) and an Immunity-Redox model (adjusted R 2 = 88.7%) were also constructed by combining the above-mentioned selected variables. The models were also cross-validated using two different sets of female mice (N = 30; N = 40). Correlation between predicted and observed life span was 0.849 (P < 0.000) for the Immunity model, 0.691 (P < 0.000) for the Redox, 0.662 (P < 0.000) for the Behavioural and 0.840 (P < 0.000) for the Immunity-Redox model. Thus, these results provide a new perspective on the use of immune function, redox and behavioural markers as prognostic tools in ageing research.