Grass carp (Ctenopharyngodon idella) RSK2 protects cells anti-apoptosis by up-regulating BCL-2. (August 2019)

• Record Type: Journal Article
• Authors: Weng, Panwei; Wu, Liping; Jiang, Zeyin; Ran, Xiaoqin; Xu, Kang; Xie, Xiaofen; Xu, Xiaowen; Chen, Xingxing; Han, Kun; Mao, Huiling; Hu, Chengyu
• Is Part Of: Developmental and comparative immunology. Volume 97(2019)
• Page Start: 45
• Page End: 56
• Publication Date: 2019-08
• Subjects: ERK1-RSK2 -- MAPK pathway -- BCL-2 -- Apoptosis -- Teleost; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2019.03.011
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: In mammals, toll-like receptor 3 (TLR3) is capable of recognizing double-stranded RNA and then initiates transcription of IFN-β . TLR3 can activate the innate immune system by phosphorylating extracellular signal-regulated kinase 1 (ERK1) in the mitogen-activated protein kinase (MAPK) pathway. As a downstream signaling protein of ERK1, ribosomal protein S6 kinase alpha 3 (RSK2) is activated through the "classical" MAPK pathway. So RSK2 plays a critical role in response to innate immune system induced by TRL3. However, the innate immune mechanism of RSK2 remains indistinct in fish. In this study, we cloned and characterized a full length cDNA sequence of RSK2 from Ctenopharyngodon idella (named CiRSK2, MH844551). The full length cDNA of CiRSK2 is 3930 bp with a coding sequence of 2202 bp encoding a polypeptide of 734 amino acids. The expression of CiRSK2 was ubiquitous and significantly up-regulated under the stimulation of poly (I:C) in eight different tissues of C. idella and C. idella kidney cells (CIK). In addition, poly (I:C) stimulation also up-regulated the expression of CiERK1 mRNA in CIK cells and the phosphorylation of CiERK1. We also demonstrated that the activated CiERK1 interacted with CiRSK2 by CO-IP assay and immunofluorescence assay. To further investigate the relationship between CiRSK2 and CiERK1, we performed subcellular localization of CiRSK2 at different periods of CiERK1 stimulation. The result showed that CiERK1 can make CiRSK2 enter the nucleus. Subsequently, we found that CiRSK2 increased the transcriptional level of CiBCL-2 and protein level of CiBCL-2 significantly. Then cell apoptosis was inhibited to a certain extent. Overall, our results suggested that CiRSK2 plays important roles in fish innate immunity and is able to inhibit cell apoptosis by up-regulating CiBCL-2. Highlights: CiERK1 and CiRSK2 responded to poly (I:C) stimulation. Poly (I:C) promoted the phosphorylation of CiERK1. Activated CiERK1 interacted with CiRSK2 and induced CiRSK2 to enter the nucleus. CiRSK2 inhibited apoptosis by up-regulating CiBCL-2 in nucleus.