Fluorescence Video Angiography for Evaluation of Dynamic Perfusion Status in an Aneurysm Preclinical Experimental Setting. Issue 4 (9th March 2019)

• Record Type: Journal Article
• Authors: Grüter, Basil Erwin; Täschler, Dominik; Rey, Jeannine; Strange, Fabio; Nevzati, Edin; Fandino, Javier; Marbacher, Serge; Coluccia, Daniel
• Is Part Of: Operative neurosurgery. Volume 17:Issue 4(2019)
• Page Start: 432
• Page End: 438
• Publication Date: 2019-03-09
• Subjects: Aneurysm -- Experimental -- Fluorescein -- Fluorescence -- Rat -- Video angiography; Nervous system -- Surgery -- Periodicals; 617.480590
• DOI: 10.1093/ons/opz011
• Languages: English
• ISSNs: 2332-4252

Abstract

Abstract: BACKGROUND: Experimental studies to assess aneurysm occlusion or perfusion typically rely on macroscopic examination or histological analysis but cannot assess dynamic perfusion. OBJECTIVE: To describe an easy-to-implement and inexpensive fluorescence angiographic technique for the in vivo assessment and imaging of the dynamic perfusion status of aneurysms and their underlying blood vessels in a rat model. METHODS: In a rat sidewall aneurysm model, the angiographic setup included 2 bandpass filters, a video camera, and a bicycle spotlight. After 48 rats underwent fluorescein angiography, dissections were performed to confirm the perfusion status by macroscopic and histologic examination of the aneurysm. RESULTS: Direct injection of 0.2 mL fluorescein 10% Faure achieved strong, clear visibility in all 48 aneurysms. Macro-/microscopic examination identified residual perfusion in 25 and complete healing in 23 aneurysms. Fluorescein imaging identified 21 of these 25 aneurysms (84%) with residual perfusion and 22 of 23 aneurysms (96%) with no residual perfusion. CONCLUSION: Our fluorescein imaging technique proved efficient for the evaluation of aneurysm patency and parent artery integrity in this experimental setting. Fluorescein is nontoxic, can be re-administered if needed, and, in this technique, can expand the armamentarium for the preclinical evaluation of dynamic perfusion status.