Combining Biomimetic Block Copolymer Worms with an Ice‐Inhibiting Polymer for the Solvent‐Free Cryopreservation of Red Blood Cells. Issue 8 (28th January 2016)

• Record Type: Journal Article
• Authors: Mitchell, Daniel E.; Lovett, Joseph R.; Armes, Steven P.; Gibson, Matthew I.
• Is Part Of: Angewandte Chemie international edition. Volume 55:Issue 8(2016)
• Page Start: 2801
• Page End: 2804
• Publication Date: 2016-01-28
• Subjects: biomaterials -- block copolymers -- cryopreservation -- micelles -- polymers; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201511454
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: The first fully synthetic polymer‐based approach for red‐blood‐cell cryopreservation without the need for any (toxic) organic solvents is reported. Highly hydroxylated block copolymer worms are shown to be a suitable replacement for hydroxyethyl starch as a extracellular matrix for red blood cells. When used alone, the worms are not a particularly effective preservative. However, when combined with poly(vinyl alcohol), a known ice‐recrystallization inhibitor, a remarkable additive cryopreservative effect is observed that matches the performance of hydroxyethyl starch. Moreover, these block copolymer worms enable post‐thaw gelation by simply warming to 20 °C. This approach offers a new solution for both the storage and transport of red blood cells and also a convenient matrix for subsequent 3D cell cultures. Abstract : Antifreeze‐free freezing : A wholly synthetic, solvent‐free approach for cellular cryopreservation is based on the use of biomimetic block copolymer worms in combination with a well‐known ice‐recrystallization inhibitor, poly(vinyl alcohol). Upon thawing, red‐blood‐cell recoveries of up to 70 % were achieved, and free‐standing hydrogels were obtained by warming to ambient temperature.