MicroRNA‐130a inhibits spermatogenesis by directly targeting androgen receptor in mouse Sertoli cells. Issue 10 (7th October 2018)

• Record Type: Journal Article
• Authors: Li, Cailing; Yang, Bo; Pan, Peng; Ma, Qian; Wu, Yong; Zhang, Zeng; Guo, Xin; Ye, Jing; Gui, Yaoting
• Is Part Of: Molecular reproduction and development. Volume 85:Issue 10(2018)
• Page Start: 768
• Page End: 777
• Publication Date: 2018-10-07
• Subjects: androgen receptor (AR) -- miR‐130a -- Sertoli cell -- spermatogenesis; Reproduction -- Periodicals; Molecular biology -- Periodicals; Molecular genetics -- Periodicals; Embryology -- Periodicals; 571.8
• DOI: 10.1002/mrd.23058
• Languages: English
• ISSNs: 1040-452X

Abstract

Abstract : MicroRNAs (miRNAs) have been shown to play a key role in spermatogenesis. However, whether the miRNAs influence androgen/androgen receptor (AR) signaling during spermatogenesis remains unclear. Using a bioinformatic approach, a potential miRNA, miR‐130a, which could bind to Ar ‐3′untranslated region directly was identified. The expression pattern of miR‐130a was further characterized by quantitative real‐time polymerase chain reaction. It was found that miR‐130a was abundant in testis and its expression level was negatively correlated with age. Overexpression of miR‐130a could inhibit AR expression both in vitro and in vivo. Moreover, the mice with an intratesticular injection of miR‐130a showed defects in spermatogenesis and increased germ cell apoptosis. Taken together, these results suggest that miR‐130a could negatively regulate AR expression in mouse Sertoli cell, which further cause defects in spermatogenesis. Abstract : MicroRNAs (miRNAs) have been shown to play a key role in the spermatogenesis. However, whether miRNAs influence androgen/androgen receptor signaling during spermatogenesis remains unclear. The current study suggested that miR‐130a disrupted spermatogenesis by directly targeting androgen receptor in mouse Sertoli cells