Comammox Nitrospira clade B contributes to nitrification in soil. (August 2019)

• Record Type: Journal Article
• Authors: Wang, Zhihui; Cao, Yanqiang; Zhu-Barker, Xia; Nicol, Graeme W.; Wright, Alan L.; Jia, Zhongjun; Jiang, Xianjun
• Is Part Of: Soil biology and biochemistry. Volume 135(2019)
• Page Start: 392
• Page End: 395
• Publication Date: 2019-08
• Subjects: Comammox -- Ammonia oxidizing bacteria -- Ammonia oxidizing archaea -- Nitrification -- DNA-SIP; Soil biochemistry -- Periodicals; Soil biology -- Periodicals; Sols -- Biochimie -- Périodiques; Sols -- Biologie -- Périodiques; Sols -- Microbiologie -- Périodiques; Bodembiologie; Biochemie; 631.46
• DOI: 10.1016/j.soilbio.2019.06.004
• Languages: English
• ISSNs: 0038-0717

Abstract

Abstract: Comammox, one nitrifying microorganism carries out the complete oxidation of ammonia to nitrate, have been recently discovered, and are found in a wide range of environments, including soil. However, conditions under which they actually contribute to nitrification in soil have not yet been demonstrated. By 13 CO2 -based DNA stable isotope probing with real-time quantitative PCR and gene sequence, we reported two uncultured strains, which are closely related to comammox Nitrospira clade B, autotrophically grew in both forest and paddy soils only in the absence of ammonium amendment. Furthermore, all clade B amoA sequences amplified from isotopically enriched genomic DNA in both soils were derived from one or two phylotypes, indicating a low diversity of active comammox strains in soils. Highlights: Demonstration of comammox growth and activity in soil. Identified conditions which preferentially select for the growth of comammox. Comammox Clade B contributes to nitrification rather than Clade A under low N conditions.