The important role of phagocytosis and interleukins for nile tilapia (Oreochromis niloticus) to defense infection of Aeromonas hydrophila based on transcriptome analysis. Issue 92 (September 2019)

• Record Type: Journal Article
• Authors: Zhang, Longgang; Wang, Chao; Liu, Han; Fu, Peisheng
• Is Part Of: Fish & shellfish immunology. Issue 92(2019)
• Page Start: 54
• Page End: 63
• Publication Date: 2019-09
• Subjects: Tilapia -- Aeromonas hydrophila -- Transcriptome -- Immune response -- Phagocytosis; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2019.05.041
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Tilapia is an important economic fish worldwide. It is vital to understand the mechanism of immune response for the prevention and treatment the infection of Aeromonas hydrophila . Based on high-throughput sequencing of Illumina HiSeq™, we found differentially expressed genes in the immune-related pathway were classified into phagosome, cytokine-cytokine receptor interaction and toll-like receptor signaling pathway. Gene Ontology terms were divided into three categories of transporting function, DNA replication activity and energy supply activity. The first one was related to phagocytosis and the process or transporting of antigen driven by tubulins; the second one was to differentiation and proliferation of lymphocyte activated by cytokines; and the former two both needed energy provided by the third one. According to colchicine assay, cross-immune assay, ELISA of interleukins and classical phagocytosis assay, phagocytosis and interleukins were verified to be most important to defense the infection of A. hydrophila. Highlights: Phagocytosis was important for tilapia against of A. hydrophila. IL-1β and IL6 were all significantly increased after infection of A. hydrophila. Phagocytosis was strengthened after reinfection of A. hydrophila.