Identification and characterization of a symbiotic agglutination-related C-type lectin from the hydrothermal vent shrimp Rimicaris exoculata. Issue 92 (September 2019)

• Record Type: Journal Article
• Authors: Liu, Xiao-Li; Ye, Sen; Cheng, Cai-Yuan; Li, Hua-Wei; Lu, Bo; Yang, Wei-Jun; Yang, Jin-Shu
• Is Part Of: Fish & shellfish immunology. Issue 92(2019)
• Page Start: 1
• Page End: 10
• Publication Date: 2019-09
• Subjects: C-type lectin -- Hydrothermal vent -- Rimicaris exoculata -- Symbiosis -- Pattern recognition receptor -- Microbial recognition; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2019.05.057
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Rimicaris exoculata (Decapoda: Bresiliidae) is one of the dominant species of hydrothermal vent communities, which inside its gill chamber harbors ectosymbioses with taxonomic invariability while compositional flexibility. Several studies have revealed that the establishment of symbiosis can be initiated and selected by innate immunity-related pattern recognition receptors (PRRs), such as C-type lectins (CTLs). In this research, a CTL was identified in R. exoculata (termed RCTL), which showed high expression at both mRNA and protein levels in the scaphognathite, an organ where the ectosymbionts are attached outside its setae. Linear correlationships were observed between the relative quantities of two major symbionts and the expression of RCTL based on analyzing different shrimp individuals. The recombinant protein of RCTL could recognize and agglutinate the cultivable γ -proteobacterium of Escherichia coli in a Ca 2+ -dependent manner, obeying a dose-dependent and time-cumulative pattern. Unlike conventional crustacean CTLs, the involvement of RCTL could not affect the bacterial growth, which is a key issue for the successful establishment of symbiosis. These results implied that RCTL might play a critical role in symbiotic recognition and attachment to R. exoculata . It also provides insights to understand how R. exoculata adapted to such a chemosynthesis-based environment. Highlights: Indispensable ectosymbionts occur in the hydrothermal vent shrimp R. exoculata . High expression of RCTL is linearly correlated with quantities of two major symbionts. rRCTL selectively recognize and agglutinate E. coli in a Ca 2+ -dependent manner. RCTL contributes to selectivity and stability of symbioses in R. exoculata .