Deriving Ligand Orientation in Weak Protein–Ligand Complexes by DEEP‐STD NMR Spectroscopy in the Absence of Protein Chemical‐Shift Assignment. (13th December 2018)

• Record Type: Journal Article
• Authors: Nepravishta, Ridvan; Walpole, Samuel; Tailford, Louise; Juge, Nathalie; Angulo, Jesus
• Is Part Of: Chembiochem. Volume 20:Number 3(2019)
• Page Start: 340
• Page End: 344
• Publication Date: 2018-12-13
• Subjects: DEEP-STD -- mixed molecular dynamics -- NMR spectroscopy -- TEMPOL; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.201800568
• Languages: English
• ISSNs: 1439-4227

Abstract

Abstract: Differential epitope mapping saturation transfer difference (DEEP‐STD) NMR spectroscopy is a recently developed powerful approach for elucidating the structure and pharmacophore of weak protein–ligand interactions, as it reports key information on the orientation of the ligand and the architecture of the binding pocket.1 The method relies on selective saturation of protein residues in the binding site and the generation of a differential epitope map by observing the ligand, which depicts the nature of the protein residues making contact with the ligand in the bound state. Selective saturation requires knowledge of the chemical‐shift assignment of the protein residues, which can be obtained either experimentally by NMR spectroscopy or predicted from 3D structures. Herein, we propose a simple experimental procedure to expand the DEEP‐STD NMR methodology to protein–ligand cases in which the spectral assignment of the protein is not available. This is achieved by experimentally identifying the chemical shifts of the residues present in binding hot‐spots on the surface of the receptor protein by using 2D NMR experiments combined with a paramagnetic probe. Abstract : A DEEPer meaning : Paramagnetic probes can be used with differential epitope mapping saturation transfer difference NMR experiments so as to provide information on ligand orientation and the architecture of the binding pocket in a protein–ligand complex even if the protein chemical shifts are unknown.