Homogeneity and stability of a secondary microbiological reference material candidate for Salmonella in fish matrix. (January 2020)

• Record Type: Journal Article
• Authors: Kurniawati, E; Ibrahim, B; Desniar,
• Is Part Of: IOP conference series. Volume 404(2020)
• Publication Date: 2020-01
• Subjects: FPH -- homogeneity -- microbiological reference material -- stability; Earth sciences -- Periodicals; Environmental sciences -- Congresses; Environmental sciences -- Periodicals; 550.5
• DOI: 10.1088/1755-1315/404/1/012036
• Languages: English
• ISSNs: 1755-1307

Abstract

Abstract: Reference material (RM) is defined as a material, sufficiently homogeneous and stable with respect to one or more specified properties which has been established to be fit for its intended use in a measurement process. RM is one of the tools used to assess laboratory performance on an ongoing basis, as quality control in conducting testing and can be used to calibrate an equipment and to validate or verify the testing method. Currently microbiological reference materials with fish matrices are not much available so that there is a need for RM with fisheries matrices specification. Comprehensive studies of homogeneity and stability are need to be able to develop of any reference material. Initially, a feasibility study revealed a suitable processing procedure for fish matrix, the fish protein hydrolysate (FPH) was prepared by enzymatic hydrolysis, freeze-drying, and milling. Microbiological reference material with fish matrix were shown to be sufficiently homogeneous, and storage at temperatures of -20°C for 4 weeks statistically showed the effect of storage time (P value that was significant at 5% significance level). These results provide the basis for the development of a RM for Salmonella in fish matrix.