Characterization of Peanut Stripe Virus from West Nusa Tenggara. (December 2019) - Julisaniah, N I
Abstract: Peanut Stripe Virus (PStV) infection on peanut plants causes a reduction in the yield of peanuts. This study was aimed to characterize PStV that infected peanut ( Arachis hypogaea ) plants in the West Nusa Tenggara province. Peanut plants that showed symptoms of stripes on leaves were collected from West Nusa Tenggara province peanut fields. Stripes symptoms on leaves indicated that the plant was infected with PStV. PStV mechanical inoculation to the healthy Chenopodium amaranticolor plant with sap from PStV infected peanut leaf showed local lesions symptoms on the leaf. Molecular characterization was carried out using RT-PCR with specific primers produced fragments of 1071 nt, consists of NIb, coat protein (CP) gene, and 3'UTR. Based on nucleotide sequences of coat protein gene, PStV from West Nusa Tenggara 98-99 % was similar with others PStV isolated reference in Genbank.