Complete Genomes of Two Novel Active Prophages Discovered by Bioinformatics Methods from High-Throughput Sequencing Data. (December 2018)

• Record Type: Journal Article
• Authors: Xie, Xiangcheng; Sun, Qiang; Liao, Xiangke; Tong, Yigang; Peng, Shaoliang
• Is Part Of: IOP conference series. Volume 466(2018)
• Publication Date: 2018-12
• Subjects: Materials science -- Periodicals; 620.1105
• DOI: 10.1088/1757-899X/466/1/012032
• Languages: English
• ISSNs: 1757-8981

Abstract

Abstract: Bacteriophages constitute key gene transfer agents in many bacteria. Specifcally, they may confer gene mobility to Staphylococcus warneri that usually dwells in the skin of human and animals. In this study, we first extracted samples of bacteria from foot skin of foot ulcers sufferers and develop these bacteria. Then, our bioinformatic methods for prophage predicting were applied to the genome sequences of Staphylococcus warneri which are assembly from high-throughput sequencing data. Two novel sequences predicted to encode complete prophages were found in the bacterial genome. Using the inducing agent mitomycin C, we produced phage suspensions. These indeed encompassed the progeny of the two identifed prophages (denoted IME1367_01 and IME1367_02). From high-throughput sequencing data of the suspensions, we obtained the two complete sequence of phage IME1367_01 and IME1367_02. By electron microscopic, we observed these two different phages. The complete genomes of IME1367_01 and IME1367_02 are summited to NCBI: "https://www.ncbi.nlm.nih.gov/nuccore/KY653124.1"&"https://www.ncbi.nlm.nih.gov/nuccor e/KY653121.1". It will enrich our understanding of the lysogenic phages of Staphylococcus warneri.