Dual-enzymatically crosslinked hyaluronic acid hydrogel as a long-time 3D stem cell culture system. (11th June 2020)

• Record Type: Journal Article
• Authors: Gao, Feng; Li, Jinrui; Wang, Luyu; Zhang, Dan; Zhang, Junni; Guan, Fangxia; Yao, Minghao
• Is Part Of: Biomedical materials. Volume 15:Number 4(2020:Aug.)
• Publication Date: 2020-06-11
• Subjects: hyaluronic acid hydrogel -- dual-enzymatically crosslinking -- long-time three-dimensional stem cell culture; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1088/1748-605X/ab712e
• Languages: English
• ISSNs: 1748-6041

Abstract

Abstract: Stem cell-based tissue engineering shows enormous potential for regenerative medicine. Three-dimensional (3D) stem cell culture is the most basic aspect of tissue engineering. However, achievement of a perfect scaffold for highly efficient 3D cell culture is currently still limited. Herein, a new hyaluronic acid hydrogel dual-enzymatically crosslinked by horseradish peroxidase and choline oxidase is developed as a 3D stem cell culture system. This hydrogel possesses superior stability over two months, controllable biodegradability with hyaluronidases, a high swelling ratio exceeding 6000%, and excellent cytocompatibility in vitro and biocompatibility in vivo . More importantly, a long-time and highly cellular activity 3D culture of bone marrow-derived mesenchymal stem cells was achieved in vitro over 20 days. All these encouraging results highlight the great potential of this new hydrogel for 3D culture and tissue engineering.