Estimation of point spread function of an optical microscope using stochastic minimization of least square errors. (28th April 2020)

• Record Type: Journal Article
• Authors: Buddha, Satya S Goutam; Boruah, Bosanta R
• Is Part Of: Journal of optics. Volume 22:Number 5(2020)
• Publication Date: 2020-04-28
• Subjects: point spread function -- point spread function estimation -- optical microscope -- deconvolution -- aberration; Optics -- Periodicals; 535.05
• DOI: 10.1088/2040-8986/ab8611
• Languages: English
• ISSNs: 2040-8978

Abstract

Abstract: In this paper we propose a point spread function (PSF) estimation scheme for an optical microscope. The scheme is based on minimizing the least square error between the image of an arbitrary target and the convolution of a stochastically constructed PSF array with the geometrical-optics predicted image of the target. The proposed scheme is independent of the geometry of the imaging system and will work in any type of optical microscopes. The scheme enables PSF estimation without requiring any modification of the microscope setup. We have demonstrated the proposed scheme both numerically and experimentally.