The CC-chemokine receptor 2 is involved in the control of ovarian folliculogenesis and fertility lifespan in mice. (September 2020)

• Record Type: Journal Article
• Authors: Santos, Alana Gessica Amaral; Pereira, Luiza Aparecida Ansaloni Chagas; Viana, Joao Henrique Moreira; Russo, Remo Castro; Campos-Junior, Paulo Henrique Almeida
• Is Part Of: Journal of reproductive immunology. Volume 141(2020)
• Publication Date: 2020-09
• Subjects: Ovary -- CCR2 -- Follicle -- Aging -- Fertility; Reproduction -- Immunological aspects -- Periodicals; Immunology -- Periodicals; Allergy and Immunology -- Periodicals; Reproduction -- Periodicals; Reproduction -- Immunologie -- Périodiques; Immunologie -- Périodiques; Immunology; Reproduction -- Immunological aspects; Periodicals; Electronic journals; Electronic journals; 615.766
• DOI: 10.1016/j.jri.2020.103174
• Languages: English
• ISSNs: 0165-0378

Abstract

Highlights: CC-chemokine Receptor 2 is expressed by oocytes included in growing follicles. CCR2 deficiency attenuates ovarian follicles activation and atresia in adult mice. Genetic ablation of CCR2 prolongs ovarian lifespan. CCR2 regulates ovarian follicular activation and atresia. Abstract: The chemokine receptor 2 (CCR2) was first described as a chemotactic factor involved in immune responses, but it also plays an essential function in several biological processes. The chemokine (C–C motif) ligand 2 (CCL2) binds to CCR2 triggering G protein-coupled receptor (GPCR) signaling in leukocytes, including activation of PI3K/Akt/mTOR, a key pathway that is also related to follicular activation and survival. However, the potential role of CCR2 in ovarian follicular physiology remain unexplored. Thus, we investigated the role of CCR2 on follicular growth during adult life and aging. Ovaries and oocytes were collected from wild type (WT) mice at 1.5 months old (mo), and CCR2 expression was observed predominantly in oocytes included in growing follicles, as well as after ovulation. Follicle populations were assessed in WT and CCR2-/- mice at 1.5 mo, and CCR2-/- mice had more primordial and less primary and secondary follicles, while there were no differences in antral follicle numbers. Pro-apoptotic genes Bax and Casp3 were downregulated, while anti-apoptotic Bcl2 was upregulated in CCR2-/- mice. To further characterize the role of CCR2 in ovarian aging, follicle populations were assessed in WT and CCR2-/- mice at 1.5, 2.5, 6, 10, and 12 mo. A larger ovarian follicular reserve at 1.5–6 mo was observed in CCR2-/- mice. Finally, CCR2-/- aged mice (6–12 mo) ovulated more oocytes than WT mice. Altogether, these data suggest that CCR2 plays an important role in the regulation of murine folliculogenesis, potentially affecting the reproductive lifespan.