Anaerobic ammonium oxidation (anammox) bacterial diversity, abundance, and activity in sediments of the Indus Estuary. (30th September 2020)

• Record Type: Journal Article
• Authors: Fozia, ; Zheng, Yanling; Hou, Lijun; Zhang, Zongxiao; Chen, Feiyang; Gao, Dengzhou; Yin, Guoyu; Han, Ping; Dong, Hongpo; Liang, Xia; Yang, Yi; Liu, Min
• Is Part Of: Estuarine, coastal and shelf science. Volume 243(2020)
• Publication Date: 2020-09-30
• Subjects: Anammox -- Nitrogen -- Sediment -- The Indus Estuary; Estuarine oceanography -- Periodicals; Coasts -- Periodicals; Estuarine biology -- Periodicals; Seashore biology -- Periodicals; Coasts; Estuarine biology; Estuarine oceanography; Seashore biology; Periodicals; 551.461805
• DOI: 10.1016/j.ecss.2020.106925
• Languages: English
• ISSNs: 0272-7714

Abstract

Abstract: Anaerobic ammonium oxidation (anammox) is an important bioprocess for nitrogen removal and has been studied in estuarine environments. However, knowledge on anammox bacterial community dynamics and related controlling factors remains limited in these ecosystems. In this study, the community compositions, abundance, and activity of anammox bacteria in the surface sediments from the Indus Estuary were investigated along a salinity gradient, considering the links between the anammox bacterial community dynamics and environmental variables. The potential importance of anammox was also estimated for nitrogen removal. High anammox bacterial diversity was detected in the sediments of the Indus Estuary, including Kuenenia, Brocadia, Scalindua, Jettenia, and a novel anammox-like cluster. Kuenenia was identified as the dominant anammox bacteria in most samples. Anammox bacterial diversity was significantly correlated with sediment NO3 −, while the distribution of anammox bacterial community was significantly related to temperature and sediment sulfide ( P < 0.05). The anammox bacterial abundance based on the 16S rRNA gene varied between 1.64 × 10 6 copies g −1 and 8.21 × 10 8 copies g −1, and was significantly correlated with sediment Fe(II). Based on an 15 N isotope-tracing technique, potential anammox rates were found in the range 0.01–0.32 μ mol N kg −1 h −1, and were controlled mainly by salinity, Fe(II), and TOC. It was estimated that the anammox bacteria contributed about 21.9% to the total nitrogen loss, on average. These results show the importance of anammox bacteria for nitrogen transformation and removal in estuarine and coastal environments. Graphical abstract: Image 1 Highlights: High diversity of anammox bacteria was detected in the estuarine environments. Anammox bacterial diversity was associated with sediment NO3 − Distribution of anammox bacteria was related to temperature and sediment sulfide. Anammox bacteria played an important role in nitrogen removal.