Effect of augmented glycation in mobilization of plasma free fatty acids in type 2 diabetes mellitus. Issue 5 (September 2020)

• Record Type: Journal Article
• Authors: Chandra, Kailash; Jain, Vineet; Azhar, Masood; Khan, Washim; Alam, Ozair; Ahmad, Sayeed; Jain, Swatantra Kumar
• Is Part Of: Diabetes & metabolic syndrome. Volume 14:Issue 5(2020)
• Page Start: 1385
• Page End: 1389
• Publication Date: 2020-09
• Subjects: Type 2 diabetes mellitus -- Free fatty acids -- Gas chromatography-mass spectrometry -- GLUT-4 -- Docking analysis; Diabetes -- Periodicals; Metabolism -- Disorders -- Periodicals; Diabetes Mellitus -- Periodicals; Metabolic Diseases -- Periodicals; Diabète -- Périodiques; Métabolisme, Troubles du -- Périodiques; Endocrinologie -- Périodiques; Diabète -- Physiopathologie -- Périodiques; Diabetes; Metabolism -- Disorders; Electronic journals; Periodicals; 616.462
• DOI: 10.1016/j.dsx.2020.07.028
• Languages: English
• ISSNs: 1871-4021

Abstract

Abstract: Background and aims: Type 2 Diabetes Mellitus (T2DM) is known to be associated with an increase in total plasma free fatty acid (FFA) concentration. The present study was conducted to determine the changes in plasma fatty acids at different levels of glycation in type 2 diabetes mellitus. Methods: The study involved 50 subjects having different levels of glycation (HbA1c 4.9–15.0%) and further categorized in 5 groups [group 1 (HbA1c <6%), group 2 (HbA1c 6–7%), group 3 (HbA1c 7.1–9%), group 4 (HbA1c (9.1–12%) and group 5 (HbA1c >12%)] with 10 subjects in each group. Results: A total of 19 free fatty acids were detected by gas chromatography-mass spectrometry (GC-MS) analysis in the plasma samples. The levels of lauric acid (C12:0) and stearic acid (C18:0) were significantly raised at an advanced stage of glycation (HbA1c 9.1–15%). Long-chain fatty acids, pentadecanoic acid (C15:0) and palmitic acid (C16:0) levels were elevated in hyperglycemia as compared to normoglycaemic subjects (HbA1c <6%). Moreover, levels of mono and polyunsaturated fatty acids, oleic acid (C18:1) and linoleic acid (C18:2, w6) were significantly decreased in patients with increased levels of glycation (HbA1c 6–15%). Conclusion : GC-MS is a novel way to study the plasma fatty acid profiling and findings of this study suggest that monitoring alterations in plasma FFA profile may be of prognostic value. Graphical abstract: Image 1 Highlights: Fatty acid profiling was carried out at different glycation levels in human subjects. Palmitic acid (C16:0) level was significantly elevated in type 2 diabetes mellitus (T2DM) patients. Stearic acid (C18:0) and margaric acid (C17:0) were positively and negatively correlated with HbA1c, respectively. Mono and polyunsaturated fatty acid levels were significantly decreased in T2DM patients.