Poly(3-hydroxybutyrate-3-hydroxyvalerate) production from pretreated waste lignocellulosic hydrolysates and acetate co-substrate. (November 2020)

• Record Type: Journal Article
• Authors: Yin, Fen; Li, Dongna; Ma, Xiaojun; Li, Jianing; Qiu, Yujuan
• Is Part Of: Bioresource technology. Volume 316(2020)
• Publication Date: 2020-11
• Subjects: Lignocellulose -- Co-substrate -- Activated sludge -- PHBV; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2020.123911
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: It was a sustainable and economical approach to produce PHBV from co-substrate. Limiting nitrogen and micro alkaline culture was more conducive to produce PHBV. The maximum yield of PHBV was 2308.45 mg/L and HV content reached 14.51%. Acidovorax was the dominant genus of all bioreactors. Abstract: The purpose of this study was to explore the potential of producing Poly(3-hydroxybutyrate-3-hydroxyvalerate) (PHBV) by mixed microbial culture (MMC) with lignocellulosic hydrolysates and acetate co-substrate as feedstock. The addition of co-substrate acetate led to the introduction of HV monomer into the polyhydroxyalkanoate (PHA), and the initial mixed sludge suspension (MLSS) increased with the increase of acetate. Almost 1.91-fold increase in the yield of PHA was achieved with limited nitrogen medium (the carbon to nitrogen ratio (C/N) was 33) compared to the normal nitrogen medium (C/N = 20). Limiting nitrogen source and micro alkaline culture environment was more conducive to the accumulation of PHBV. PHA production achieved to the highest value of about 2308.45 mg/L under the condition of optimized technology. Acidovorax was the dominant genus of all bioreactors using co-substrate. Further, utilizing lignocellulosic hydrolysate and acetate co-substrate as feedstock in mixed microbial culture was a promising approach in a low-cost large-scale PHA production.