Comparison of QuantiFERON-TB Gold Plus, QuantiFERON-TB Gold In-Tube, and T-SPOT.TB among patients with tuberculosis. Issue 11 (November 2020)

• Record Type: Journal Article
• Authors: Takeda, Keita; Nagai, Hideaki; Suzukawa, Maho; Sekiguchi, Ryo; Akashi, Shunsuke; Sato, Ryota; Narumoto, Osamu; Kawashima, Masahiro; Suzuki, Junko; Ohshima, Nobuharu; Yamane, Akira; Tamura, Atsuhisa; Matsui, Hirotoshi; Tohma, Shigeto
• Is Part Of: Journal of infection and chemotherapy. Volume 26:Issue 11(2020:Nov.)
• Page Start: 1205
• Page End: 1212
• Publication Date: 2020-11
• Subjects: Latent TB infection -- Previous tuberculosis -- Interferon (IFN)-γ release assay; LTBI latent tuberculosis infection -- NHO National Hospital Organization -- IFN interferon -- IGRAs IFN-γ release assays -- QFT QuantiFERON -- QFT-GIT QFT-Gold In-Tube -- QFT-Plus QFT-TB Gold Plus -- TB tuberculosis; Chemotherapy -- Periodicals; Infection -- Periodicals; Communicable diseases -- Chemotherapy -- Periodicals; 615.5805
• DOI: 10.1016/j.jiac.2020.06.019
• Languages: English
• ISSNs: 1341-321X

Abstract

Abstract: Objectives: This study evaluated the efficacy of the following interferon (IFN)-γ release assays (IGRAs): QuantiFERON-TB Gold Plus (QFT-Plus), QFT-Gold In-Tube (QFT-GIT), and T-SPOT. TB (T-SPOT) with the quantitative values of IFN-γ response. Methods: Blood samples were collected from patients with active tuberculosis (TB), latent TB infection (LTBI), individuals with previous TB infection, and healthy volunteers enrolled between May 2017 and June 2018. Results: IGRAs results were analyzed in 175 subjects (76 had active TB, 14 had LTBI, 35 had prior TB infection, and 50 were healthy). QFT-Plus and QFT-GIT revealed equal efficacy for IFN-γ values, and the IFN-γ response in QFTs tended to increase with the spot counts in T-SPOT, with similar high sensitivities (approximately 90%) in the active TB group. The test concordance of two of three IGRAs was optimal among all subjects (κ coefficients: 0.82–0.96). Additionally, the median quantitative values of IFN-γ with QFT-Plus and QFT-GIT were higher in the active TB group than in the LTBI and previous TB groups. Conclusion: Three IGRAs showed equivalent efficacy with high sensitivities and higher IFN-γ response in active TB group than that in non-active TB group.