The fabrication and characterization of bioengineered ultra-high molecular weight polyethylene-collagen-hap hybrid bone-cartilage patch. (September 2020)

• Record Type: Journal Article
• Authors: Kan, Yuliya; Cvjetinovic, Julijana; Statnik, Eugene S.; Ryazantsev, Sergey V.; Anisimova, Natalia Yu.; Kiselevskiy, Mikhail V.; Salimon, Alexey I.; Maksimkin, Alexey V.; Korsunsky, Alexander M.
• Is Part Of: Materials today communications. Volume 24(2020)
• Publication Date: 2020-09
• Subjects: Collagen -- Hydroxyapatite -- Freeze-casting -- Micromechanical bending test -- Electron microscopy -- UHMWPE; Materials science -- Periodicals; 620.11
• DOI: 10.1016/j.mtcomm.2020.101052
• Languages: English
• ISSNs: 2352-4928

Abstract

Graphical abstract: Highlights: A glue-free junction of layers enhances the structure of the bulk and porous bioinert ultra-high weight polyethylene (UHMWPE) through the biodegradable collagen derived layer maintaining strong adhesion. Attenuated Total Reflectance Fourier transform infrared (ATR FTIR) spectroscopy of collagen helps optimize the fabrication of the bone-cartilage patch. Abstract: A layered hybrid implant was designed and fabricated for the surgical replacement of worn cartilage to meet the complex requirements of biocompatibility and mechanics. The natural hierarchical structure was purposefully mimicked to improve the implant performance and integration. The hybrid was fabricated using three components: processed collagen gel, hydroxyapatite (HAp) powder and ultra-high molecular weight polyethylene (UHMWPE) in bulk and sponge form. The fabrication included hot molding, sacrificial templating, infusion, and freeze-casting stages. The hybrid had a porous transition layer made of porous UHMWPE impregnated with collagen by means of infusion. SEM and FTIR analyses confirmed successful collagen impregnation of porous UHMWPE. The morphology of transition layer was selected and produced to provide the UHMWPE pore size distribution in a range ∼50–150 μm that is favorable for the osseointegration by osteoblast proliferation. Biocompatibility tests were carried out in vitro. The index of red blood cells hemolysis showed 0.6 % after 4 h of co-incubation that proved excellent biocompatibility of the fabricated UHMWPE-Collagen-HAp hybrid.