Ratiometric fluorescence In3+ sensing via In3+-triggered tautomerization: Its applications to water samples, live cells and zebrafish. (December 2020)

• Record Type: Journal Article
• Authors: Yun, Dongju; Chae, Ju Byeong; So, Haeri; Do, Trinh N.; Kim, Ki-Tae; Yi, Yelim; Lim, Mi Hee; Kim, Cheal
• Is Part Of: Dyes and pigments. Volume 183(2020)
• Publication Date: 2020-12
• Subjects: Indium -- Ratiometric -- Fluorescence chemosensor -- Tautomerization -- Calculations -- Bioimaging; Dyes and dyeing -- Periodicals; Pigments -- Periodicals; 667.2
• DOI: 10.1016/j.dyepig.2020.108704
• Languages: English
• ISSNs: 0143-7208

Abstract

Abstract: A novel ratiometric fluorescence sensor NCS ((E)-N-(furan-2-ylmethyl)-2-(2-hydroxybenzylidene)hydrazine-1-carbothioamide) was synthesized by a combination of furfuryl isothiocyanate bearing hydrazine and salicylaldehyde. Sensor NCS exhibited a ratiometric fluorescent response toward In 3+ via thioamide tautomerization with blue-shifted emission from 484 to 444 nm in a near-perfect aqueous solution. NCS displayed a linear ratiometric relationship to micromolar concentrations (0–60 μ M) of In 3+ and the detection limit was found to be 2.68 μ M. Moreover, sensor NCS exhibited an excellent selectivity for sensing In 3+ in real samples as well as live cell and zebrafish. The sensing mechanism was demonstrated through UV–visible, fluorescent and 1 H NMR titrations, Job's plot, ESI-MS and theoretical calculations. Graphical abstract: Image 1 Highlights: A ratiometric fluorescent sensor NCS was developed for detection of In 3+ at neutral pH. NCS exhibited ratiometric fluorescent response toward In 3+ via thioamide tautomerization. NCS could detect In 3+ in living organisms such as live cells and zebrafish, for the first time. Sensing mechanism of In 3+ was demonstrated by the spectroscopic studies and calculations.