Hemicyanine-based colorimetric and near-infrared fluorescent off-on probe for Hg2+ detection and imaging in living cells and zebrafish. (December 2020)

• Record Type: Journal Article
• Authors: Yuan, Gang; Lv, Hehong; Liu, Heng; He, Hanping; Sun, Qi; Zhang, Xiuhua; wang, Shengfu
• Is Part Of: Dyes and pigments. Volume 183(2020)
• Publication Date: 2020-12
• Subjects: NIR fluorescent probe -- Hg2+ -- Thiocarbonate -- Imaging -- Colorimetric; Dyes and dyeing -- Periodicals; Pigments -- Periodicals; 667.2
• DOI: 10.1016/j.dyepig.2020.108674
• Languages: English
• ISSNs: 0143-7208

Abstract

Abstract: Herein, a novel near-infrared fluorescent probe (Cy-PT) for the detection of Hg 2+ was designed and synthesized, which contained a hemicyanine skeleton as the near-infrared (NIR) fluorophore and carbonothioate moiety as the recognition unit. The very weak fluorescence of Cy-PT is greatly enhanced due to the destruction of thiocarbonate moiety by Hg 2+, generating a hydroxyl group. The study shows that Cy-PT exhibits many advantages including NIR emission (708 nm), colorimetric change, off-on fluorescence, remarkably large Stokes shift (120 nm), rapid response, excellent sensitivity and high selectivity. In addition, Cy-PT can be used as a direct visualization detector for Hg2+ detection, allowing further application using test paper. Subsequent fluorescence imaging shows that Cy-PT probe has high ability to enter living cells and bodies, generating strong NIR fluorescence response in the presence of Hg 2+ . This study indicates that Cy-PT probe is a good candidate for Hg 2+ detection in colorimetric and near-infrared fluorescence, which can be developed into an efficient method for Hg 2+ detection in complex living samples. Graphical abstract: Image 1 Highlights: A novel probe (Cy-PT) for detecting Hg 2+ was designed and synthesized. Cy-PT exhibits many advantages including NIR emission (708 nm), colorimetric change, off-on fluorescence, remarkably large Stokes shift (120 nm), rapid response, excellent sensitivity and high selectivity. Cy-PT probe has high ability to enter living cells and bodies, generating strong NIR fluorescence response in the presence of Hg 2+ .