Boron analysis and imaging of cells with 2-hr BPA exposure by using micro-proton particle-induced gamma-ray emission (PIGE). (November 2020)

• Record Type: Journal Article
• Authors: Nakai, Kei; Endo, Keita; Yoshida, Fumiyo; Koka, Masashi; Yamada, Naoto; Satoh, Takahiro; Tsurubuchi, Takao; Matsumura, Akira; Matsumoto, Yoshitaka; Sakurai, Hideyuki
• Is Part Of: Applied radiation and isotopes. Volume 165(2020:Nov.)
• Publication Date: 2020-11
• Subjects: Boron neutron capture therapy -- Particle induced gamma-ray emission -- Boron imaging -- Boronophenylalanine; Radiology -- Periodicals; Radiation -- Industrial applications -- Periodicals; Nuclear chemistry -- Periodicals; Internet resource; Periodical; 660.298
• DOI: 10.1016/j.apradiso.2020.109334
• Languages: English
• ISSNs: 0969-8043

Abstract

Abstract: Particle-induced gamma-ray emission (PIGE), which measures prompt gamma rays at 428 keV from 10 B (p, p'γ) 7 Be, was used to confirm the boron distribution within 2hr-BPA-exposed cells. Distribution images of potassium, phosphate, and boron and the whole spectrum showed the ratios of boron counts to total (%) as follows: control group: 1.35 ± 0.073%; 2hr boron exposure group: 2.33 ± 0.35%; and boron exposure/wash group: 1.58 ± 0.095%. Micro-beam PIXE/PIGE can be a promising tool for visualization of intracellular Boron. Highlights: Particle-induced gamma-ray emission confirmed cellular boron distribution. Micro-PIGE Gamma ray counts rate was well correlated with boron concentration. Micro-beam PIXE/PIGE can be a promising tool for visualizing intracellular Boron dynamics.