Interaction of the plasmid-encoded quinolone resistance protein QnrB19 with Salmonella Typhimurium DNA gyrase. Issue 11 (November 2020)

• Record Type: Journal Article
• Authors: Pachanon, Ruttana; Koide, Kentaro; Kongsoi, Siriporn; Nakajima, Chie; Kapalamula, Thoko Flav; Suthienkul, Orasa; Suzuki, Yasuhiko
• Is Part Of: Journal of infection and chemotherapy. Volume 26:Issue 11(2020:Nov.)
• Page Start: 1139
• Page End: 1145
• Publication Date: 2020-11
• Subjects: PMQR -- Quinolone resistance -- QnrB19 -- Salmonella typhimurium -- DNA gyrase; Chemotherapy -- Periodicals; Infection -- Periodicals; Communicable diseases -- Chemotherapy -- Periodicals; 615.5805
• DOI: 10.1016/j.jiac.2020.06.002
• Languages: English
• ISSNs: 1341-321X

Abstract

Abstract: Background: Plasmid-encoded quinolone resistance protein Qnr is an important factor in bacterial resistance to quinolones. Qnr interacts with DNA gyrase and reduces susceptibility to quinolones. The gene qnr likely spreads rapidly among Enterobacteriaceae via horizontal gene transfer. Though the vast amounts of epidemiological data are available, molecular details of the contribution of QnrB19, the predominant Qnr in Salmonella spp., to the acquisition of quinolone resistance has not yet been understood well . Objective: We aimed to examine the role of QnrB19 in quinolone resistance acquisition using recombinant Salmonella Typhimurium DNA gyrases and QnrB19. Materials and methods: Recombinant QnrB19 was expressed in E. coli and purified by Ni-NTA agarose column chromatography. DNA supercoiling activities of recombinant Salmonella Typhimurium DNA gyrase were assessed with or without QnrB19 under the existence of three quinolones to measure IC50 s, the concentration of each quinolone required for 50% inhibition in vitro . Results: The IC50 s of norfloxacin, ciprofloxacin and nalidixic acid against DNA gyrases were measured to be 0.30, 0.16 and 17.7 μg/mL, respectively. The addition of QnrB19 increased the IC50 s of norfloxacin and ciprofloxacin to be 0.81 and 0.48 μg/mL, respectively, where no effect of QnrB19 was observed on the IC50 of nalidixic acid. Conclusion: QnrB19 was shown for the first time in vitro to have ability to grant non-classical quinolone resistance to S. Typhimurium DNA gyrase. Structural insight on quinolones in this study may contribute to investigate drugs useful for preventing the spread of plasmid carrying PMQR along with other factors associating with antimicrobial resistance in S. Typhimurium and other bacteria.