TGF-β3 suppresses melanogenesis in human melanocytes cocultured with UV-irradiated neighboring cells and human skin. Issue 2 (August 2020)

• Record Type: Journal Article
• Authors: Moon, Hye-Rim; Jung, Joon Min; Kim, Su Yeon; Song, Youngsup; Chang, Sung Eun
• Is Part Of: Journal of dermatological science. Volume 99:Issue 2(2020)
• Page Start: 100
• Page End: 108
• Publication Date: 2020-08
• Subjects: α-MSH α-melanocyte stimulating hormone -- DMEM Dulbecco's modified Eagle's medium -- ET-1 endothelin-1 -- ERK extracellular signal-regulated kinase -- FBS fetal bovine serum -- FBs human dermal fibroblasts -- GSK glycogen synthase kinase -- KCs keratinocytes -- MCs melanocytes -- MITF microphthalmia-associated transcription factor -- NHMs normal human melanocytes -- SCF stem cell factor -- TGF transforming growth factor -- TGFBRI TGF-β receptor I -- TGFBRII TGF-β receptor II -- TRP tyrosinase related protein -- UVR ultraviolet radiation; TGF-β3 -- Melanogenesis -- UV radiation -- Human skin; Dermatology -- Periodicals; Skin Diseases -- Periodicals; Dermatologie -- Périodiques; 616.5005
• DOI: 10.1016/j.jdermsci.2020.06.007
• Languages: English
• ISSNs: 0923-1811

Abstract

Highlights: TGF-β3 decreases melanin content in UVB-treated melanocyte-keratinocyte-fibroblast coculture. TGF-β3 reduces melanin content in UVB-treated ex vivo human skin. TGF-β3 decreases melanogenesis via the ERK pathway. Abstract: Background: Ultraviolet radiation (UVR) is the most well-known cause of skin pigmentation accompanied with photoaging. Transforming growth factor (TGF)-β1 was previously shown to have anti-melanogenic property; however, it can induce scarring in skin. Objective: We investigated the effect of TGF-β3 on melanogenesis in human melanocytes cocultured with UV-irradiated skin constituent cells, and UV-irradiated human skin. Methods: UVB irradiation or treatment with stem cell factor (SCF) and endothelin-1 (ET-1) was applied to human melanocytes cocultured with keratinocytes and/or fibroblasts and ex vivo human skin. Mechanistic pathways were further explored after treatment with TGF-β3. Results: While UVB irradiation or SCF/ET-1 enhanced melanogenesis, TGF-β3 effectively inhibited melanin accumulation and tyrosinase activity via downregulation of the extracellular signal-regulated kinase (ERK)/microphthalmia-associated transcription factor (MITF) pathway. TGF-β3 increased the expression of differentiation markers of keratinocytes. Conclusion: TGF-β3 effectively suppressed UVR-stimulated melanogenesis indicating that topical TGF-β3 may be a suitable candidate for the treatment of UV-associated hyperpigmentation disorders.