An efficient protocol for the preparation of linear arabino-oligosaccharides. (October 2020)

• Record Type: Journal Article
• Authors: Shi, Huimin; Xu, Jialei; Wang, Wenqing; Jia, Mengdi; Zhou, Yifa; Sun, Lin
• Is Part Of: Carbohydrate research. Volume 496(2020)
• Publication Date: 2020-10
• Subjects: Arabino-oligosaccharides -- Mild acid hydrolysis -- Degree of polymerization; Carbohydrates -- Periodicals; Chemistry, Organic -- Periodicals; Biochemistry -- Periodicals; Carbohydrates -- Periodicals; Chimie organique -- Périodiques; Glucides -- Périodiques; Biochemistry; Carbohydrates; Chemistry, Organic; Periodicals; Electronic journals; 507.78
• DOI: 10.1016/j.carres.2020.108131
• Languages: English
• ISSNs: 0008-6215

Abstract

Abstract: In this study, we report the development of an efficient protocol in the preparation of linear arabino-oligosaccharides derived from sugar beet arabinan. By optimizing hydrolytic conditions and separation on tandem Bio-Gel P4 columns, we obtained arabino-oligosaccharides with various degrees of polymerization (DP) from 2 to 15. All of these α-1, 5-linked arabino-oligosaccharides are highly pure (>95%) as determined by HPAEC, MALDI-TOF mass spectrometry and 13 C NMR spectroscopy. Due to their purity, these oligosaccharides can be used as standards to identify other oligosaccharides and as substrates to characterize new arabinan-specific enzymes, as well as for the development of new functional oligosaccharides. Graphical abstract: Image 1 Highlights: An efficient protocol was developed to prepare linear arabino-oligosaccharides. A series of arabino-oligosaccharides with DP 2 to 15 were produced. They were characterized by HPAEC, MALDI-TOF MS and 13 C NMR spectroscopy. These oligosaccharides were all in the form of α-1, 5-linkages with high purity.