CircHIPK3 regulates melanoma cell behaviors by binding with miR-215–5p to upregulate YY1. (October 2020)

• Record Type: Journal Article
• Authors: Zhu, Xiaomei; Sun, Jianfang
• Is Part Of: Molecular and cellular probes. Volume 53(2020)
• Publication Date: 2020-10
• Subjects: circHIPK3 -- miR-215–5p -- YY1 -- Melanoma; Molecular probes -- Diagnostic use -- Periodicals; Pathology, Cellular -- Technique -- Periodicals; Cell Biology -- Periodicals; Molecular Biology -- Periodicals; Sondes moléculaires -- Utilisation diagnostique -- Périodiques; Cytopathologie -- Technique -- Périodiques; 572
• DOI: 10.1016/j.mcp.2020.101644
• Languages: English
• ISSNs: 0890-8508

Abstract

Abstract: Object: To investigate the role of circHIPK3 in melanoma. Methods: Bioinformatics analysis and experiments including RT-qPCR, Pearson's correlation analysis, luciferase reporter, Western blot, and RIP assays were applied to explore the function and mechanism of circHIPK3 in melanoma. Results: CircHIPK3 expression was strikingly upregulated while miR-215–5p was downregulated in melanoma tissues and cell lines. Pearson's correlation analysis unveiled circHIPK3 expression was positively correlated with Ki-67 (a marker of proliferation), which implied that circHIPK3 may play a vital role in the progression of melanoma. In mechanism, luciferase reporter and RIP assays validated that circHIPK3 was able to bind with miR-215–5p. Moreover, we confirmed that overexpression of circHIPK3 could facilitate cell proliferation and depress cell apoptosis in melanoma while overexpression of miR-215–5p exerted opposite effects. Besides, our findings indicated that miR-215–5p overexpression significantly reversed the circHIPK3 overexpressing-mediated promotive effect on cell proliferation and inhibitory effect on cell apoptosis. Furthermore, we found that miR-215–5p could directly target YY1. Upregulation of YY1 could notably offset the inhibitory effect of circHIPK3 downregulation on cell proliferation and the promotive effect on cell apoptosis. Conclusion: Our study corroborated that circHIPK3 regulated melanoma cell behaviors via the miR-215–5p/YY1 axis, which might provide a novel insight for the treatment of melanoma patients. Highlights: CircHIPK3 presents high expression in melanoma. CircHIPK3 sponges miR-215–5p in melanoma. CircHIPK3 promotes cell proliferation in melanoma by sponging miR-215–5p. CircHIPK3 suppresses cell apoptosis in melanoma by sponging miR-215–5p.