Actinomyces wuliandei sp. nov., Corynebacterium liangguodongii sp. nov., Corynebacterium yudongzhengii sp. nov. and Oceanobacillus zhaokaii sp. nov., isolated from faeces of Tibetan antelope in the Qinghai-Tibet plateau of China. Issue 6 (4th June 2020)

• Record Type: Journal Article
• Authors: Zhu, Wentao; Li, Junqin; Wang, Xiaoxia; Yang, Jing; Lu, Shan; Lai, Xin-He; Jin, Dong; Huang, Yuyuan; Zhang, Sihui; Pu, Ji; Zhou, Juan; Ren, Zhihong; Huang, Ying; Wu, Xiaomin; Xu, Jianguo
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 70:Issue 6(2020)
• Page Start: 3763
• Page End: 3774
• Publication Date: 2020-06-04
• Subjects: Actinomyces wuliandei -- Corynebacterium liangguodongii -- Corynebacterium yudongzhengii -- Oceanobacillus zhaokaii -- Tibetan antelope -- Qinghai-Tibet Plateau; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.004232
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : Eight Gram-stain-positive, rod-shaped bacterial strains were isolated from faeces of Tibetan antelopes on the Tibet-Qinghai Plateau of China. Genomic sequence analysis showed that the strains belong to the genera Actinomyces (strains 299 T and 340), Corynebacterium (strains 2184 T, 2185, 2183 T and 2189) and Oceanobacillus (strains 160 T and 143), respectively, with a percentage of similarity for the 16S rRNA gene under the species threshold of 98.7 % except for strains 160 T and 143 with Oceanobacillus arenosus CAU 1183 T (98.8 %). The genome sizes (and genomic G+C contents) were 3.1 Mb (49.4 %), 2.5 Mb (64.9 %), 2.4 Mb (66.1 %) and 4.1 Mb (37.1 %) for the type strains 299 T, 2183 T, 2184 T and 160 T, respectively. Two sets of the overall genome relatedness index values between our isolates and their corresponding closely related species were under species thresholds (95 % for average nucleotide identity, and 70 % for digital DNA–DNA hybridization). These results, together with deeper genotypic, genomic, phenotypic and biochemical analyses, indicate that these eight isolates should be classified as representing four novel species. Strain 299 T (=CGMCC 1.16320 T =JCM 33611 T ) is proposed as representing Actinomyces wuliandei sp. nov.; strain 2184 T (=CGMCC 1.16417 T =DSM 106203 T ) is proposed as representing Corynebacterium liangguodongii sp. nov.; strain 2183 T (=CGMCC 1.16416 T =DSM 106264 T ) is proposed as representing Corynebacterium yudongzhengii sp. nov.; and strain 160 T (=CGMCC 1.16367 T =DSM 106186 T ) is proposed as representing Oceanobacillus zhaokaii sp. nov.