Caproicibacter fermentans gen. nov., sp. nov., a new caproate-producing bacterium and emended description of the genus Caproiciproducens. Issue 7 (25th July 2020)

• Record Type: Journal Article
• Authors: Flaiz, Maximilian; Baur, Tina; Brahner, Sven; Poehlein, Anja; Daniel, Rolf; Bengelsdorf, Frank R.
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 70:Issue 7(2020)
• Page Start: 4269
• Page End: 4279
• Publication Date: 2020-07-25
• Subjects: strictly anaerobic -- Gram-stain-negative -- spore-forming -- Caproiciproducens; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.004283
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A strictly anaerobic bacterial strain designated EA1 T was isolated from an enrichment culture inoculated with biogas reactor content. Cells of strain EA1 T are spore-forming rods (1–3×0.4–0.8 µm) and stain Gram-negative, albeit they possess a Gram-positive type of cell-wall ultrastructure. Growth of strain EA1 T was observed at 30 and 37 °C and within a pH range of pH 5–9. The major components recovered in the fatty acid fraction were C14:0, C16:0, C16:0 DMA (dimethyl acetal) and C16:1 ω 7 c . Strain EA1 T fermented several mono- and disaccharides. Metabolic end products from fructose were acetate, butyrate, caproate and lactate. Furthermore, ethanol, CO2 and H2 were identified as products. The genome consists of a chromosome (3.9 Mbp) with 3797 predicted protein-encoding genes and a G+C content of 51.25 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain EA1 T represents a novel taxon within the family Oscillospiraceae . The most closely related type strains of EA1 T, based on 16S rRNA gene sequence identity, are Caproiciproducens galactitolivorans BS-1 T (94.9 %), [ Clostridium ] leptum DSM 753 T (93.8 %), [ Clostridium ] sporosphaeroides DSM 1294 T (91.7 %) and Ruminococcus bromii ATCC 27255 T (91.0 %). Further phenotypic characteristics of strain EA1 T differentiate it from related, validly described bacterial species. Strain EA1 T represents a novel genus and novel species within the family Oscillospiraceae . The proposed name is Caproicibacter fermentans gen. nov., sp. nov. The type strain is EA1 T (DSM 107079 T =JCM 33110 T ).