Polaribacter septentrionalilitoris sp. nov., isolated from the biofilm of a stone from the North Sea. Issue 7 (24th July 2020)

• Record Type: Journal Article
• Authors: Choo, Simeon; Borchert, Erik; Wiese, Jutta; Saha, Mahasweta; Künzel, Sven; Weinberger, Florian; Hentschel, Ute
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 70:Issue 7(2020)
• Page Start: 4305
• Page End: 4314
• Publication Date: 2020-07-24
• Subjects: Polaribacter septentrionalilitoris -- North Sea -- polyphasic taxonomy; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.004290
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A new member of the family Flavobacteriaceae was isolated from the biofilm of a stone at Nordstrand, a peninsula at the German North Sea shore. Phylogenetic analysis of the 16S rRNA gene sequence showed that strain ANORD1 T was most closely related to the validly described type strains Polaribacter porphyrae LNM-20 T (97.0 %) and Polaribacter reichenbachii KMM 6386 T (96.9 % 16S rRNA gene sequence similarity) and clustered with Polaribacter gangjinensis K17-16 T (96.0 %). Strain ANORD1 T was determined to be mesophilic, Gram-negative, non-motile and strictly aerobic. Optimal growth was observed at 20–30 °C, within a salinity range of 2–7 % sea salt and from pH 7–10. Like other type strains of the genus Polaribacter, ANORD1 T was tested negative for flexirubin-type pigments, while carotenoid-type pigments were detected. The DNA G+C content of strain ANORD1 T was 30.6 mol%. The sole respiratory quinone detected was menaquinone 6 (MK-6). The major fatty acids identified were C15 : 0, iso-C15 : 0, C15 : 1 ω6 c and iso-C15 : 0 3-OH. Based on the polyphasic approach, strain ANORD1 T represents a novel species in the genus Polaribacter, with the name Polaribacter septentrionalilitoris sp. nov. being proposed. The type strain is ANORD1 T (=DSM 110039 T =NCIMB 15081 T =MTCC 12685 T ).