Corynebacterium urogenitale sp. nov. isolated from the genital tract of a cow. Issue 6 (27th June 2020)

• Record Type: Journal Article
• Authors: Ballas, Panagiotis; Rückert, Christian; Wagener, Karen; Drillich, Marc; Kämpfer, Peter; Busse, Hans-Jürgen; Ehling-Schulz, Monika
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 70:Issue 6(2020)
• Page Start: 3625
• Page End: 3632
• Publication Date: 2020-06-27
• Subjects: bovine -- Corynebacterium -- uterine sampling; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.004198
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A Gram-stain-positive bacterial isolate, designated LMM-1652 T, was isolated from an intrauterine cytobrush sample originating from a postpartum Holstein Friesian dairy cow. The strain had a rod to coccoid-shape, was catalase-positive and oxidase-negative. 16S rRNA gene sequence similarity analyses revealed that its closest relatives were Corynebacterium falsenii (97.05 % similarity), Corynebacterium jeikeium (96.83 %) and Corynebacterium urealyticum (96.82 %). Subsequent whole genome analysis showed that the genome-to-genome distance of strain LMM-1652 T to its closest relatives was in the range of 23.2–24.8 %, while the average nucleotide identity values ranged from 73.7 to 74.3%, thus confirming that this isolate represents a novel species. Strain LMM-1652 T was characterized by a quinone system mainly consisting of MK-9(H2 ) and MK-10(H2 ). The polar lipids profile of the strain consisted mainly of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol-mannoside, as well as one unidentified lipid lacking any functional group. Smaller amounts of four unidentified phospholipids, four unidentified glycolipids, β-gentiobiosyl diacylglycerol and four unidentified lipids lacking a functional group were also found. The cell wall contained meso -diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan. The fatty acid profile was mainly composed of C18 : 1 ω9 c, C18 : 0 and C16 : 0 . We propose a novel species of the genus Corynebacterium with the name Corynebacterium urogenitale LMM-1652 T (=LMG 31163 T =DSM 108747 T ).