Flavobacterium salilacus sp. nov., isolated from surface water of a hypersaline lake, and descriptions of Flavobacterium salilacus subsp. altitudinum subsp. nov. and Flavobacterium salilacus subsp. salilacus subsp. nov. Issue 7 (22nd July 2020)

• Record Type: Journal Article
• Authors: Debnath, Sanjit Chandra; Chen, Can; Khan, Ishrat; Wang, Wen-Jie; Zheng, Dao-Qiong; Xu, Jin-Zhong; Wang, Pin-Mei
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 70:Issue 7(2020)
• Page Start: 4250
• Page End: 4260
• Publication Date: 2020-07-22
• Subjects: Flavobacterium -- polyphasic analysis -- novel subspecies -- hypersaline lake -- genome sequence; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.004281
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : Two yellow-pigmented, Gram-stain-negative, aerobic, rod-shaped bacteria were isolated from the water of the hypersaline Chaka Salt Lake (strain SaA2.12 T ) and sediment of Qinghai Lake (strain LaA7.5 T ), PR China. According to the 16S rRNA phylogeny, the isolates belong to the genus Flavobacterium, showing the highest 16S rRNA sequence similarities to Flavobacterium arcticum SM1502 T (97.6–97.7 %) and Flavobacterium suzhouense XIN-1 T (96.5–96.6 %). Moreover, strains SaA2.12 T and LaA7.5 T showed 99.73 % 16S rRNA sequence similarity to each other. Major fatty acids, respiratory quinones and polar lipids detected in these isolates were iso-C15 : 0, menaquinone-6 and phosphatidylethanolamine, respectively. Strains SaA2.12 T and LaA7.5 T showed significant unique characteristics between them as well as between the closest phylogenetic members. The highest digital DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) values between SaA2.12 T and its closest neighbours were 25.3 and 82.8 %, respectively; whereas these values (highest) between LaA7.5 T and its closest members were 25.2 and 82.8 %, respectively. The dDDH and ANI values between strains SaA2.12 T and LaA7.5 T were calculated as 75.9 and 97.2 %, respectively. Therefore, based on polyphasic data, we propose that strain SaA2.12 T represents a novel species with the name Flavobacterium salilacus sp. nov., with the type strain SaA2.12 T (=KCTC 72220 T =MCCC 1K03618 T ) and strain LaA7.5 T as a subspecies within novel Flavobacterium salilacus with the name Flavobacterium salilacus subsp. altitudinum subsp. nov., with the type strain LaA7.5 T (=KCTC 72806 T =MCCC 1K04372 T ). These propositions automatically create Flavobacterium salilacus subsp. salilacus subsp. nov. with SaA2.12 T (=KCTC 72220 T =MCCC 1K03618 T ) as the type strain.