Salinicoccus cyprini sp. nov., isolated from the gut of mirror carp, Cyprinus carpio var. specularis. Issue 7 (15th July 2020)

• Record Type: Journal Article
• Authors: Talwar, Chandni; Singh, Amit Kumar; Choksket, Stanzin; Korpole, Suresh; Lal, Rup; Negi, Ram Krishan
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 70:Issue 7(2020)
• Page Start: 4111
• Page End: 4118
• Publication Date: 2020-07-15
• Subjects: Salinicoccus -- taxonomy -- taxo-genomics; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.004247
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A novel orange to pink coloured bacterial strain designated as CT19 T was isolated from the gastrointestinal tract of mirror carp, Cyprinus carpio var. specularis (Lacepède, 1803) collected from the Gobind Sagar reservoir at village Lathiani, Una, Himachal Pradesh, India. Cells of the strain were found to be aerobic, Gram-stain-positive, non-motile and non-spore-forming coccoids. Based on the 16S rRNA gene sequence, the strain was closely related to Salinicoccus hispanicus J-82 T (=DSM 5352 T ; 97.4 %), followed by S. sesuvii CC-SPL15-2 T (=DSM 23267 T ; 96.4 %), S. amylolyticus JC304 T (=KCTC 33661 T ; 95.6 %) and S. roseus DSM 5351 T (95.4 %). Identity with all other members of the genus were <94.5 %. The draft genome of strain CT19 T was assembled to 2.4 Mbp with a G+C content of 47.9 mol%. Average nucleotide identity and digital DNA–DNA hybridization values between strain CT19 T and S. hispanicus J-82 T were found to be 85.9 and 31.3% respectively which is far below the threshold for species delineation. Iso-C15 : 0, anteiso-C15 : 0, iso-C17 : 0, C16 : 0 and anteiso-C17 : 0 were the major cellular fatty acids of strain CT19 T . Major polar lipids were diphosphatidylglycerol, phosphatidylgylcerol and an unidentified glycolipid. Respiratory quinone system was composed of menaquinone-6 and major cell wall amino acid was l -lysine. Based on phylogenomic, physiological and biochemical characteristics, strain CT19 T represents a novel species of the genus Salinicoccus for which the name Salinicoccus cyprini sp. nov. is proposed. The type strain is CT19 T (=KCTC 43022 T =CCM 8886 T =MCC 3834 T ).