Chitinophaga tropicalis sp. nov., isolated from forest soil. Issue 6 (5th June 2020)

• Record Type: Journal Article
• Authors: Zhang, Xian-Jiao; Feng, Guang-Da; Yao, Qing; Zhang, Jun; Phuong, Le Thi Bich; Zhu, Honghui
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 70:Issue 6(2020)
• Page Start: 3859
• Page End: 3864
• Publication Date: 2020-06-05
• Subjects: Chitinophaga tropicalis -- forest soil -- whole genome sequence -- dDDH -- ANI; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.004248
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A novel bacterial strain, designated ysch24 T, was isolated from a forest soil sample collected from the Cat Tien National Park, southern Vietnam. Cells were Gram-stain-negative, aerobic, gliding, filamentous or rod-shaped. The results of 16S rRNA gene analyses revealed that strain ysch24 T belongs to the genus Chitinophaga, and was most closely related to Chitinophaga silvisoli GDMCC 1.1411 T (97.4 %), followed by Chitinophaga oryziterrae JCM 16595 T (97.3 %) and Chitinophaga sancti NBRC 15057 T (96.9 %). The average nucleotide identity and digital DNA–DNA hybridization values between strain ysch24 T and closely related type strains were 72.0–74.0 % and 19.1–19.4 %, respectively. Major fatty acids were iso-C15 : 0, C16 : 1 ω5 c and iso-C17 : 0 3-OH and the predominant respiratory quinone was menaquinone 7. Polar lipids consisted of phosphatidylethanolamine, four unidentified aminophospholipids, two unidentified phospholipids and four unidentified lipids. The genomic DNA G+C content was 45.6 mol%. The study clearly showed that strain ysch24 T should represent a novel species of the genus Chitinophaga, for which the name Chitinophaga tropicalis sp. nov. is proposed. The type strain is ysch24 T (=GDMCC 1.1355 T =KACC 21527 T ).