Pseudomonas yangonensis sp. nov., isolated from wound samples of patients in a hospital in Myanmar. Issue 6 (5th June 2020)

• Record Type: Journal Article
• Authors: Tohya, Mari; Watanabe, Shin; Teramoto, Kanae; Tada, Tatsuya; Kuwahara-Arai, Kyoko; Mya, San; Zin, Khwar Nyo; Kirikae, Teruo; Tin, Htay Htay
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 70:Issue 6(2020)
• Page Start: 3597
• Page End: 3605
• Publication Date: 2020-06-05
• Subjects: Pseudomonas -- human pathogen -- Pseudomonas oleovorans group; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.004181
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : Strains of a Gram-negative, aerobic, rod-shaped, non-spore-forming bacterium, designated MY50 T, MY63 and MY101, were isolated from wound samples of three hospitalized patients in Yangon, Myanmar. Strains MY50 T, MY63 and MY101 grew at temperatures of 4–44 °C, in media containing 1.0–7.0 % (w/v) NaCl and at pH 6.0–9.5. Phylogenetic analysis based on 16S rRNA gene and whole genome sequences showed that these strains belonged to the genus Pseudomonas and were part of the Pseudomonas oleovorans group and located close to Pseudomonas guguanensis and Pseudomonas mendocina . Whole-genome comparisons, using average nucleotide identity and digital DNA–DNA hybridization analyses, confirmed that strains MY50 T, MY63 and MY101 were the same strain and they were a distinct species in the P. oleovorans group. Results of phenotypic characterization tests demonstrated that utilization of p-hydroxy-phenylacetic acid, glycerol, l -pyroglutamic acid and quinic acid could distinguish these strains from other species of the P. oleovorans group. These genetic and phenotypic characteristics suggest that they should be classified as representing a novel species, under the proposed name Pseudomonas yangonensis sp. nov. The type strain is MY50 T (=LMG 31602 T, =JCM 33396 T ), with a DNA G+C content of 62.82 mol%.