Mimovirus Vesicle‐Based Biological Orthogonal Reaction for Cancer Diagnosis. Issue 9 (18th June 2020)

• Record Type: Journal Article
• Authors: Ren, En; Chu, Chengchao; Zhang, Yunming; Wang, Junqing; Pang, Xin; Lin, Xiaoning; Liu, Chao; Shi, Xiaoxiao; Dai, Qixuan; Lv, Peng; Wang, Xiaomin; Chen, Xiaoyuan; Liu, Gang
• Is Part Of: Small methods. Volume 4:Issue 9(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-06-18
• Subjects: biological orthogonal reaction -- cancer diagnosis -- cell membrane vesicles -- low‐pH activated fusion -- tumor heterogeneity; Nanotechnology -- Methodology -- Periodicals; Nanotechnology -- Periodicals; Periodicals; 620.5028
• DOI: 10.1002/smtd.202000291
• Languages: English
• ISSNs: 2366-9608

Abstract

Abstract: Herein, a tumor microenvironment (TME)‐responsive mimovirus vesicle (MVV) is engineered to deliver azide motifs (–N3 ) via the membrane fusion mechanism for cancer diagnosis. As a pH‐responsive functional protein, the spike vesicular stomatitis virus G‐protein (VSVG) is genetically immobilized on surface of cell membrane vesicles. By virtue of the low‐pH activated fusogenic peculiarity of VSVG, the –N3 groups, which are also presented on MVVs via metabolic engineering, can be directly anchored onto the target cells, thus reducing tumor heterogeneity and serving as the bait to amplify the tumor targeting of dibenzocyclooctyne‐modified diagnostic moieties. The potential diagnostic capability of such design is successfully confirmed in three different murine tumor models. Featuring excellent pH‐sensitive fusogenic traits, the developed MVVs show great responsiveness to the slightly acidic TME of solid tumors, providing a universal platform in overcoming tumor heterogeneity for precise cancer diagnosis. Abstract : Benefiting from spike vesicular stomatitis virus G‐protein and azide motifs (–N3 ) presented herein, tumor acidic microenvironment‐responsive mimovirus vesicles can deploy –N3 to the target cell surface via the membrane fusion mechanism without considering the tumor heterogeneity. The artificially transplanted –N3 can serve as the bait to amplify the tumor‐targeting ability of dibenzocyclooctyne‐modified moieties for cancer diagnosis.