In situ isobaric lipid mapping by MALDI–ion mobility separation–mass spectrometry imaging. (21st June 2020)

• Record Type: Journal Article
• Authors: Fu, Tingting; Oetjen, Janina; Chapelle, Manuel; Verdu, Alexandre; Szesny, Matthias; Chaumot, Arnaud; Degli‐Esposti, Davide; Geffard, Olivier; Clément, Yohann; Salvador, Arnaud; Ayciriex, Sophie
• Is Part Of: Journal of mass spectrometry. Volume 55:Number 9(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-06-21
• Subjects: ion mobility separation -- lipid isomers -- MALDI -- mass spectrometry imaging -- TIMS; Mass spectrometry -- Periodicals; 543.65
• DOI: 10.1002/jms.4531
• Languages: English
• ISSNs: 1076-5174

Abstract

Abstract: The highly diverse chemical structures of lipids make their analysis directly from biological tissue sections extremely challenging. Here, we report the in situ mapping and identification of lipids in a freshwater crustacean Gammarus fossarum using matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) in combination with an additional separation dimension using ion mobility spectrometry (IMS). The high‐resolution trapped ion mobility spectrometry (TIMS) allowed efficient separation of isobaric/isomeric lipids showing distinct spatial distributions. The structures of the lipids were further characterized by MS/MS analysis. It is demonstrated that MALDI MSI with mobility separation is a powerful tool for distinguishing and localizing isobaric/isomeric lipids.