Endosomal Escape of Polymer‐Coated Silica Nanoparticles in Endothelial Cells. Issue 36 (9th July 2020)

• Record Type: Journal Article
• Authors: Parodi, Alessandro; Evangelopoulos, Michael; Arrighetti, Noemi; Cevenini, Armando; Livingston, Megan; Khaled, Sm Z.; Brown, Brandon S.; Yazdi, Iman K.; Paradiso, Francesca; Campa‐Carranza, Jocelyn N.; De Vita, Alessandro; Taraballi, Francesca; Tasciotti, Ennio
• Is Part Of: Small. Volume 16:Issue 36(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-07-09
• Subjects: drug delivery -- endosomal escape -- endothelial cells, nanoparticles -- nanosafety; Nanotechnology -- Periodicals; Nanoparticles -- Periodicals; Microtechnology -- Periodicals; 620.5
• DOI: 10.1002/smll.201907693
• Languages: English
• ISSNs: 1613-6810

Abstract

Abstract: Current investigations into hazardous nanoparticles (i.e., nanotoxicology) aim to understand the working mechanisms that drive toxicity. This understanding has been used to predict the biological impact of the nanocarriers as a function of their synthesis, material composition, and physicochemical characteristics. It is particularly critical to characterize the events that immediately follow cell stress resulting from nanoparticle internalization. While reactive oxygen species and activation of autophagy are universally recognized as mechanisms of nanotoxicity, the progression of these phenomena during cell recovery has yet to be comprehensively evaluated. Herein, primary human endothelial cells are exposed to controlled concentrations of polymer‐functionalized silica nanoparticles to induce lysosomal damage and achieve cytosolic delivery. In this model, the recovery of cell functions lost following endosomal escape is primarily represented by changes in cell distribution and the subsequent partitioning of particles into dividing cells. Furthermore, multilamellar bodies are found to accumulate around the particles, demonstrating progressive endosomal escape. This work provides a set of biological parameters that can be used to assess cell stress related to nanoparticle exposure and the subsequent recovery of cell processes as a function of endosomal escape. Abstract : Smart materials have attracted significant attention due to their ability to modulate the surrounding environment. However, in the biological context, they cannot be presumed inert. A set of parameters to assess biological function following endosomal disruption and escape of polymer‐coated silica nanoparticles is provided. This analysis provides insight into the recovery of endothelial cells following nanoparticle insult.