Jaburetox, a urease‐derived peptide: Effects on enzymatic pathways of the cockroach Nauphoeta cinerea. Issue 2 (6th August 2020)

• Record Type: Journal Article
• Authors: Perin, Ana P. A.; Noronha, Mila S.; Moyetta, Natalia R.; Coste Grahl, Matheus V.; Fruttero, Leonardo L.; Staniscuaski, Fernanda
• Is Part Of: Archives of insect biochemistry and physiology. Volume 105:Issue 2(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-08-06
• Subjects: AChE -- action mechanism -- CNS -- Jaburetox -- Nauphoeta cinerea -- nitric oxide synthase -- UAP; Insects -- Physiology -- Periodicals; Insect biochemistry -- Periodicals; 595.701572
• DOI: 10.1002/arch.21731
• Languages: English
• ISSNs: 0739-4462

Abstract

Abstract: Jaburetox is a recombinant peptide derived from one of the Canavalia ensiformis urease isoforms. This peptide induces several toxic effects on insects of different orders, including interference on muscle contractility in cockroaches, modulation of UDP‐ N ‐acetylglucosamine pyrophosphorylase (UAP) and nitric oxide synthase (NOS) activities in the central nervous system of triatomines, as well as activation of the immune system in Rhodnius prolixus . When injected, the peptide is lethal for R. prolixus and Triatoma infestans . Here, we evaluated Jaburetox toxicity to Nauphoeta cinerea cockroaches, exploring the effects on the central nervous system through the activities of UAP, NOS, acid phosphatases (ACP), and acetylcholinesterase (AChE). The results indicated that N. cinerea is not susceptible to the lethal effect of the peptide. Moreover, both in vivo and in vitro treatments with Jaburetox inhibited NOS activity, without modifying the protein levels. No alterations on ACP activity were observed. In addition, the enzyme activity of UAP only had its activity affected at 18 hr after injection. The peptide increased the AChE activity, suggesting a mechanism involved in overcoming the toxic effects. In conclusion, our findings indicate that Jaburetox affects the nitrinergic signaling as well as the AChE and UAP activities and establishes N. cinerea as a Jaburetox‐resistant model for future comparative studies. Abstract : JBTX, Jaburetox; CNS, central nervous system; NOS, nitric oxide synthase; UAP, UDP‐ N ‐acetylglucosamine pyrophosphorylase; ACP, acid phosphatase; AChE, acetylcholinesterase. Research Highlights: 1. The in vivo treatment with Jaburetox (Jbtx) did not compromise the survival of the cockroaches. 2. Jbtx affects the nitrinergic signaling of a nonhemipteran species. 3. The findings allow the establishment of Nauphoeta cinerea as a Jbtx‐resistant model.