Validation of diffuse correlation spectroscopy against 15O-water PET for regional cerebral blood flow measurement in neonatal piglets. Issue 10 (October 2020)

• Record Type: Journal Article
• Authors: Giovannella, Martina; Andresen, Bjørn; Andersen, Julie B; El-Mahdaoui, Sahla; Contini, Davide; Spinelli, Lorenzo; Torricelli, Alessandro; Greisen, Gorm; Durduran, Turgut; Weigel, Udo M; Law, Ian
• Is Part Of: Journal of cerebral blood flow & metabolism. Volume 40:Issue 10(2020)
• Page Start: 2055
• Page End: 2065
• Publication Date: 2020-10
• Subjects: Cerebral blood flow -- PET -- diffuse correlation spectroscopy -- near-infrared spectroscopy -- continuous neuro-monitoring; Cerebral circulation -- Periodicals; Brain -- Metabolism -- Periodicals; Brain -- Blood-vessels -- Periodicals; Cerebrovascular disease -- Periodicals; 612.824
• DOI: 10.1177/0271678X19883751
• Languages: English
• ISSNs: 0271-678X

Abstract

Diffuse correlation spectroscopy (DCS) can non-invasively and continuously asses regional cerebral blood flow (rCBF) at the cot-side by measuring a blood flow index (BFI) in non-traditional units of cm 2 /s. We have validated DCS against positron emission tomography using 15 O-labeled water ( 15 O-water PET) in a piglet model allowing us to derive a conversion formula for BFI to rCBF in conventional units (ml/100g/min). Neonatal piglets were continuously monitored by the BabyLux device integrating DCS and time resolved near infrared spectroscopy (TRS) while acquiring 15 O-water PET scans at baseline, after injection of acetazolamide and during induced hypoxic episodes. BFI by DCS was highly correlated with rCBF (R = 0.94, p < 0.001) by PET. A scaling factor of 0.89 (limits of agreement for individual measurement: 0.56, 1.39)×10 9 × (ml/100g/min)/(cm 2 /s) was used to derive baseline rCBF from baseline BFI measurements of another group of piglets and of healthy newborn infants showing an agreement with expected values. These results pave the way towards non-invasive, cot-side absolute CBF measurements by DCS on neonates.