Peri-Infarct Hot-Zones Have Higher Susceptibility to Optogenetic Functional Activation-Induced Spreading Depolarizations. Issue 8 (August 2020)

• Record Type: Journal Article
• Authors: Sugimoto, Kazutaka; Chung, David Y.; Böhm, Maximilian; Fischer, Paul; Takizawa, Tsubasa; Aslihan Aykan, Sanem; Qin, Tao; Yanagisawa, Takeshi; Harriott, Andrea; Oka, Fumiaki; Yaseen, Mohammad A.; Sakadžić, Sava; Ayata, Cenk
• Is Part Of: Stroke. Volume 51:Issue 8(2020)
• Publication Date: 2020-08
• Subjects: cerebral ischemia -- laser speckle imaging -- middle cerebral artery occlusion -- migraine aura -- optogenetics; Cerebrovascular disease -- Periodicals; Cerebral circulation -- Periodicals; 616.81
• DOI: 10.1161/STROKEAHA.120.029618
• Languages: English
• ISSNs: 0039-2499

Abstract

Abstract : Background and Purpose: Spreading depolarizations (SDs) are recurrent and ostensibly spontaneous depolarization waves that may contribute to infarct progression after stroke. Somatosensory activation of the metastable peri-infarct tissue triggers peri-infarct SDs at a high rate. Methods: We directly measured the functional activation threshold to trigger SDs in peri-infarct hot zones using optogenetic stimulation after distal middle cerebral artery occlusion in Thy1-ChR2-YFP mice. Results: Optogenetic activation of peri-infarct tissue triggered SDs at a strikingly high rate (64%) compared with contralateral homotopic cortex (8%; P =0.004). Laser speckle perfusion imaging identified a residual blood flow of 31±2% of baseline marking the metastable tissue with a propensity to develop SDs. Conclusions: Our data reveal a spatially distinct increase in SD susceptibility in peri-infarct tissue where physiological levels of functional activation are capable of triggering SDs. Given the potentially deleterious effects of peri-infarct SDs, the effect of sensory overstimulation in hyperacute stroke should be examined more carefully. Abstract : Supplemental Digital Content is available in the text.