Glyphosate-based herbicide has soil-mediated effects on potato glycoalkaloids and oxidative status of a potato pest. (November 2020)

• Record Type: Journal Article
• Authors: Rainio, Miia J.; Margus, Aigi; Virtanen, Valtteri; Lindström, Leena; Salminen, Juha-Pekka; Saikkonen, Kari; Helander, Marjo
• Is Part Of: Chemosphere. Volume 258(2020)
• Publication Date: 2020-11
• Subjects: Antioxidant defense -- Herbivores -- Insects -- Potato defense chemicals -- Roundup -- α-solanine; CAT catalase -- GBH glyphosate-based herbicide -- GP glutathione peroxidase -- GR glutathione reductase -- GSH glutathione -- GSH:GSSG reduced vs. oxidized form of glutathione -- GST glutathione-S-transferase -- LHP lipid hydroperoxides -- ROS reactive oxygen species -- SOD superoxide dismutase -- tGSH total glutathione; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2020.127254
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Glyphosate is the most used herbicide worldwide, targeting physiological pathways in plants. Recent studies have shown that glyphosate can also cause toxic effects in animals. We investigated the glyphosate-based herbicide (GBH)-induced changes in potato ( Solanum tuberosum ) plant chemistry and the effects of a GBH on the survival rate and oxidative status of the Colorado potato beetle ( Leptinotarsa decemlineata ). The beetles were reared on potato plants grown in pots containing soil treated with a GBH (Roundup Gold, 450 g/l) or untreated soil (water control). The 2nd instar larvae were introduced to the potato plants and then collected in 2 phases: as 4th instar larvae and as adults. The main glycoalkaloids of the potato plants, α-solanine and α-chaconine, were measured twice during the experiment. The α-solanine was reduced in potato plants grown in GBH-treated soil, which can be detrimental to plant defenses against herbivores. GBH treatment had no effect on the survival rate or body mass of the larvae or the adult beetles. In the larvae, total glutathione (tGSH) concentration and the enzyme activity of catalase (CAT), superoxide dismutase, and glutathione-S-transferase were increased in the GBH treatment group. In the adult beetles, CAT activity and tGSH levels were affected by the interactive effect of GBH treatment and the body mass. To conclude, environmentally relevant concentrations of a GBH can affect the potato plant's glycoalkaloid concentrations, but are not likely to directly affect the survival rate of the Colorado potato beetle, but instead, modify the antioxidant defense of the beetles via diet. Graphical abstract: Image 1 Highlights: The α-solanine levels were reduced in potato plants grown in GBH-treated soil. The survival of the beetles was not affected by the soil-mediated GBH treatment. Indirect GBH treatment modify the antioxidant defense of the Colorado potato beetle larvae. Soil-mediated GBH treatment at larval stage may have long-term effects on the adult beetles.