Comprehensive RNA-Seq profiling of the lung transcriptome of Argali hybrid sheep in response to experimental Mycoplasma ovipneumoniae infection. (October 2020)

• Record Type: Journal Article
• Authors: Li, Zengqiang; Du, Zhihui; Sun, Yanming; Wang, Jixue; Liu, Haiyan; Yang, Yi; Zhao, Ning
• Is Part Of: Research in veterinary science. Volume 132(2020)
• Page Start: 57
• Page End: 68
• Publication Date: 2020-10
• Subjects: Argali hybrid sheep -- Mycoplasma ovipneumoniae -- Lung -- Transcriptomics -- Hyper-IgM syndrome; MO Mycoplasma ovipneumoniae -- DPI Day Post Infection -- CCU Color change unit -- DEGs differentially expressed genes -- KEGG Kyoto Encyclopedia of Genes and Genomes -- GO Gene Ontology -- CAMs Cell adhesion molecules -- SPLUNC1 short palate, lung and nasal epithelium clone 1; Veterinary medicine -- Periodicals; Veterinary Medicine -- Periodicals; Médecine vétérinaire -- Périodiques; Médecine vétérinaire -- Recherche -- Périodiques; Diergeneeskunde; 636.089
• DOI: 10.1016/j.rvsc.2020.05.014
• Languages: English
• ISSNs: 0034-5288

Abstract

Abstract: Background: An experiment was conducted to reveal why the Argali hybrid sheep are susceptible to Mycoplasma ovipneumoniae infection, the causative agent of mycoplasma ovipneumonia, a chronic respiratory disease that is harmful to the sheep industry. Results: After nine Argali hybrid sheep, divided into three groups, were experimentally infected with an M. ovipneumoniae strain at 0, 4 and 14 days, transcriptome profiling of lung tissues was performed by deep RNA sequencing, using the Illumina platform. Analysis of differentially expressed genes was performed to determine concomitant gene-specific temporal patterns of mRNA expression in the lungs after M. ovipneumoniae infection. 156 differentially expressed genes (44 up-regulated, 112 down-regulated) were found when comparing transcriptomic data at 4 and 0 days post-infection, and 367 (35 up-regulated, 332 down-regulated) when comparing 14 versus 0 days post-infection. Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that the differentially expressed genes at 4 and 14 versus 0 days post-infection were enriched in 109 and 150 pathways, respectively, and the Primary immunodeficiency pathway was considered most closely related to MO infection ( p < .01). Hyper-IgM syndrome was identified based on the B-cell Immunodeficiency signaling pathway from differentially expressed genes related to M. ovipneumoniae infection. Gene Ontology analysis showed that differentially expressed genes in different groups were enriched for 497 and 928 terms, where those most closely related to M. ovipneumoniae infection are ciliated motor damage ( p < .01). Conclusions: The situation that ciliary movement is significantly inhibited and B cells in immunodeficiency are possibly the most important reason why Argali hybrid sheep are susceptible to MO. Highlights: Mycoplasma ovipneumonia is a highly contagious respiratory disease caused by Mycoplasma ovipneumoniae (MO). MO has a higher infection and lethality rate in sheep which are 1–3 months old. Argali hybrid sheep are susceptible to MO. In this study, the first transcriptomic analysis of sheep infected with MO was performed using high-throughput sequencing. The Primary immunodeficiency pathway was considered most closely related to MO infection.